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Michael Smith Laboratories presents Dr. Kathryn A. Whitehead

May 16, 2018 - 11:00am

Michael Smith Laboratories

2185 East Mall
Vancouver, BC, Canada, V6T 1Z4

102 (Auditorium)


Lipid-like materials for RNA delivery: A How-to Guide for Hacking Gene Expression
Dr. Kathryn A. Whitehead
Assistant Professor Departments of Chemical Engineering and Biomedical Engineering, Carnegie Mellon University, USA
Dr. Kathryn A. Whitehead

Hosted by Dr. Christian Kastrup

Despite the promise of RNA therapeutics, progress towards the clinic has been slowed by the difficulty of delivering RNA drugs, such as short interfering RNA (siRNA) and messenger RNA (mRNA), into cellular targets within the body. RNA therapeutics are large (104 – 106 g/mol) and negatively charged; they do not have favorable biodistribution properties in vivo nor an ability to cross the cellular membrane of target cells. In response to these challenges, our lab has created biodegradable, ionizable lipid-like materials called ‘lipidoids’ that readily formulate into nanoparticles containing RNA. Lipidoids efficiently manipulate gene expression in a variety of biological systems, including hepatocytes, white blood cells and tumors. This talk will focus on the cell-free prediction of lipidoid efficacy in delivering mRNA to mice. Furthermore, I will describe a new formulation strategy for the synergistic co-delivery of mRNA and siRNA. Specifically, we found that siRNA-mediated gene silencing and mRNA-mediated protein expression improved 2-3 fold when they co-formulated in the same nanoparticle. Together, these data demonstrate the potential of lipidoid materials to robustly manipulate gene expression in vivo.