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Publications - Bohlmann

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2018

Chiu CC, Keeling CI, and Bohlmann J. Monoterpenyl esters in juvenile mountain pine beetle and sex-specific release of the aggregation pheromone trans-verbenol. PNAS. doi: 10.1073/pnas.1722380115.

Parent GJ, Giguère I, Mageroy M, Bohlmann J, and MacKay JJ. Evolution of the biosynthesis of two hydroxyacetophenones in plants. Plant, Cell & Environment. doi: 10.1111/pce.13134.

Mafu S, Ding Y, Murphy KM, Yaacoobi O, Addison JB, Wang Q, Shen Z, Briggs SP, Bohlmann J, Castro-Falcón G, Hughes CC, Betsiashvili M, Huffaker A, Schmelz EA, and Zerbe P. Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize. Plant Physiology. doi: 10.1104/pp.17.01351.


2017

Celedon JM, Yuen MMS, Chiang A, Henderson H, Reid KE, and Bohlmann J. Cell-type and tissue specific transcriptomes of the white spruce (Picea glauca) bark unmask fine-scale spatial patterns of constitutive and induced conifer defense. The Plant Journal. doi: 10.1111/tpj.13673.

Mageroy MH, Jancsik S, Yuen MMS, Fischer M, Withers SG, P aetz C, Schneider B, and Bohlmann J. A conifer UDP-sugar dependent glycosyltransferase contributes to acetophenone metabolism and defense against insects. Plant Physiology. doi: 10.1104/pp.17.00611

Chiu CC, Keeling CI, and Bohlmann J. Toxicity of pine monoterpenes to mountain pine beetle. Scientific Reports. doi: 10.1038/s41598-017-08983-y

Mageroy MH, Lachance D, Jancsik S, Parent G, Séguin A, MacKay J, and Bohlmann J. In vivo function of Pgßglu-1 in the release of acetophenones in white spruce. PeerJ. doi: 10.7717/peerj.3535

Celedon JM and Bohlmann J. An extended model of heartwood secondary metabolism informed by functional genomics. Tree Physiology. doi: 10.1093/treephys/tpx070

Moniodis J, Jones CG, Renton M, Plummer JA, Barbour EL, Ghisalberti EL, and Bohlmann J. Sesquiterpene variation in West Australian Sandalwood (Santalum spicatum). Molecules. doi:10.3390/molecules22060940

Burke J, Bohlmann J, and A Carroll. Consequences of distributional asymmetry in a warming environment: invasion of novel forests by the mountain pine beetle. Ecosphere. doi: 10.1002/ecs2.1778

Booth JK, Page JE and Bohlmann J. Terpene synthases from Cannabis sativa. PLoS ONE. doi:10.1371/journal.pone.0173911

Pavy N, Lamothe M, Pelgas B, Gagnon F, Birol I, Bohlmann J, Mackay J, Isabel N and Bousquet, J. A high resolution reference genetic map positioning 8.8K genes for the conifer white spruce: Structural genomics implications and correspondence with physical distance. Plant J. doi:10.1111/tpj.13478

Mafu S, Karunanithi PS, Palazzo TA, Harrod BL, Rodriguez SM, Mollhoff IN, O’Brien TE, Tong S, Fiehn O, Tantillo DJ, Bohlmann J and Zerbe P. Biosynthesis of the microtubule-destabilizing diterpene pseudolaric acid B from golden larch involves an unusual diterpene synthase. PNAS. doi: 10.1073/pnas.1612901114

Pelot KA, Mitchell R, Kwon M, Hagelthorn DM, Wardman JF, Chiang A, Bohlmann J, Ro DK and Zerbe P. Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase. Plant J. doi:10.1111/tpj.13427

2016

Coombe L, Warren RL, Yang C, Vandervalk BP, Mayo M, Moore RA, Pleasance S, Coope RJ, Bohlmann J, Holt RA, Jones SJM, and I Birol. Assembly of the complete Sitka spruce chloroplast genome using 10X Genomics’ GemCode sequencing data. PLoS ONE. doi:10.1371/journal.pone.0163059

Whitehill JGA, Henderson H, Strong W, Jaquish B, Bohlmann J. 2016. Function of Sitka spruce stone cells as a physical defense against white pine weevil. Plant, Cell and Environment. doi: 10.1111/pce.12810.

Robert JA, Bonnett T, Pitt C, Spooner LJ, Fraser J, Yuen MMS, Keeling CI, Bohlmann J, Huber DPW. 2016. Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development. PeerJ. doi: 10.7717/peerj.2109. 

Celedon JM, Bohlmann J. 2016. Genomics-Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in Sandalwood oil Biosynthesis. Methods in Enzymology. doi: 10.1016/bs.mie.2016.03.008.

Celedon JM, Chiang A, Yuen MM, Diaz-Chavez ML, Madilao LL, Finnegan PM, Barbour EL, Bohlmann J. 2016. Heartwood specific transcriptome and metabolite signatures of tropical sandalwood (Santalum album) reveal the final step of (Z)-santalol fragrance biosynthesis. The Plant Journal. doi: 10.1111/tpj.13162.

Geisler, K, Jensen NB, Yuen MMS, Madilao L, Bohlmann J. 2016. Modularity of Conifer Diterpene Resin Acid Biosynthesis: P450 Enzymes of Different CYP720B Clades Use Alternative Substrates and Converge on the Same Products. Plant Physiology. doi:10.1104/pp.16.00180.

Jackman SD, Warren RL, Gibb EA, Vandervalk BP, Mohamadi H, Chu J, Raymond A, Pleasance S, Coope R, Wildung MR, Ritland CE, Bousquet  J, Jones, SJM, Bohlmann J, Birol I. 2016. Organellar Genomes of White Spruce (Picea glauca): Assembly and annotation. Genome Biology and Evolution. doi: 10.1093/gbe/evv244.

Andersen-Ranberg J, Kongstad KT, Nielsen MT, Jensen NB, Pateraki I, Bach SS, Hamberger B, Zerbe P, Staerk D, Bohlmann J, Møller BL, Hamberger B. 2016. Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis. Angewandte chemie. doi: 10.1002/ange.201510650.

Keeling CI, Li M, Sandhu HK, Henderson H, Yuen MMS, Bohlmann J. 2016.Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis. Insect Biochemistry and Molecular Biology. doi:10.1016/j.ibmb.2016.01.002.

2015

Whitehill JGA, Henderson H, Schuetz M, Skyba O, Yuen MMS, King J, Samuels AL, Mansfield SD, Bohlmann J. 2015. Histology and cell wall biochemistry of stone cells in the physical defense of conifers against insects. Plant, Cell and Environment. doi: 10.1111/pce.12654.

Zerbe P, Rodriguez SM, Mafu S, Chiang A, Sandhu HK, O'Neil-Johnson M, Starks CM, Bohlmann J. 2015. Exploring diterpene metabolism in non-model species: Transcriptome-enabled discovery and functional characterization of labda-7, 13E-dienyl diphosphate synthase from Grindelia robusta. The Plant Journal. doi: 10.1111/tpj.12925.

Warren RL, Keeling CI, Yuen MMS, Raymond A, Taylor GA, Vandervalk BP, Mohamadi H, Paulino D, Chiu Readman, Jackman SD, Robertson G, Yang C, Hoffmann M, Weigel D, Nelson DR, Ritland C, Isabel N, Jaquish B, Yanchuk A, Bousquet J, Jones SJM, MacKay J, Birol I, Bohlmann J. 2015.  Improved white spruce (Picea glauca) genome assemblies and annotation of large gene families of conifer terpenoid and phenolic defense metabolism. The Plant Journal. doi: 10.1111/tpj.12886

Zerbe P and Bohlmann J. 2015. Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering. Trends in Biotechnology. Elsevier, online, doi:10.1016/j.tibtech.2015.04.006.

Schrader J and Bohlmann J. 2015. Editors. Advances in biochemical engineering/biotechnology: biotechnology of isoprenoids. Springer Berlin Heidelberg, online, doi:10.1007/10_2015_308.

Zerbe P and Bohlmann J. 2015. Enzymes for synthetic biology of ambroxide-related diterpenoid fragrance compounds. In Advances in biochemical engineering/biotechnology: biotechnology of isoprenoids. Springer Berlin Heidelberg, online, doi:10.1007/10_2015_308.

Bohlmann J, Gesell A, Blaukopf M, Madilao L, Yuen MMS, Withers SG, Mattsson J, Russell JH. 2015. The gymnosperm cytochrome P450 CYP750B1 catalyzes stereospecific monoterpene hydroxylation of (+)-sabinene in thujone biosynthesis in Thuja plicata. Plant Physiology, 168 (1), 94-106. doi: 10.1104/pp.14.00315.

Moniodis J, Jones CG, Barbour EL, Plummer JA, Ghisalberti EL, Bohlmann J. 2015. The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum). Phytochemistry, online. doi:10.1016/j.phytochem.2014.12.009.

Mageroy MH, Parent G, Germanos G, Giguère I, Delvas N, Maaroufi H, Bauce É, Bohlmann J, MacKay J. 2015. Expression of the β‐glucosidase gene Pgβglu‐1 underpins natural resistance of white spruce against spruce budworm. The Plant Journal 81 (1), 68-80. doi:10.1111/tpj.12699.

Novak M, Lah L, Šala M, Stojan J, Bohlmann J, Komel R. 2015. Oleic Acid Metabolism via a Conserved Cytochrome P450 System-Mediated ω-Hydroxylation in the Bark Beetle-Associated Fungus Grosmannia clavigera. PloS One 10 (3). doi: 10.1371/journal.pone.012011.

2014

Külheim C, Jones CG, Plummer JA, Ghisalberti EL, Barbour L, Bohlmann J. 2014. Foliar application of methyl jasmonate does not increase terpenoid accumulation, but weakly elicits terpenoid pathway genes in sandalwood (Santalum album L.) seedlings. Plant Biotechnology 31 (5), 585-591. doi:10.5511/plantbiotechnology.14.1014a

Appel HM, Fescemyer H, Ehlting J, Weston D, Rehrig E, Joshi T, Xu D, Bohlmann J, Schultz J. 2014. Transcriptional responses of Arabidobsis thaliana to chewing and sucking insect herbivores. Frontiers in Plant Science 5 : 556. doi: 10.3389/fpls.2014.00565.

Pitt C, Robert JA, Bonnett TR, Keeling CI, Bohlmann J, Huber DPW. 2014. Proteomics Indicators of the Rapidly Shifting Physiology from Whole Mountain Pine Beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), Adults during Early Host Colonization. PloS One 9 (10) e110673. doi: 10.1371/journal.pone.0110673.

De La Torre A, Birol I, Bousquet J, Ingvarsson P, Jansson S, Jomes SJM, Keeling CI, MacKay J, Nilsson O, Ritland K, Street N, Yanchuk A, Zerbe P, Bohlmann J. 2014. Insights into Conifer Giga-Genomes. Plant Physiology 166 (4), 1724-1732. doi:

Jackman SD, Bohlmann J, Birol I. 2014. UniqTag: Content-derived unique and stable identifiers for gene annotation. BioRxIV, doi: .

Roach C, Hall DE, Zerbe P, Bohlmann J. 2014. Plasticity and evolution of (+)-3-Carene Synthase and (-)-Sabinene Synthase functions of a Sitka Spruce monoterpene synthase gene family associated with weevil resistance. Journal of Biological Chemistry 289 (34), 23859-23869. doi: .

Zerbe P, Chiang A, Dullat H, O-Neil-Johnson M, Starks C, Hamberger B, Bohlmann J. 2014. Diterpene synthases of the biosynthetic system of medicinally active diterpenoids in Marrubium vulgare. The Plant Journal 79 (6), 914-927. doi: 10.1111/tpj.12589.

Wang Y, Lim L, Madilao L, Lah L, Bohlmann J, Breuil C. 2014. Gene discovery for enzymes involved in limonene modification or utilization by the mountain pine beetle-associated pathogen Grosmannia clavigera. Applied and Environmental Microbiology. AEM: 00670-14.

Zerbe P, Bohlmann J. 2014. Bioproducts, Biofuels, and Perfumes: Conifer Terpene Synthases and their Potential for Metabolic Engineering. In  Phytochemicals - Biosynthesis, Function and Application. R. Jetter (Ed.). Springer Publications: 85-107.

Janes JK, Li Y, Keeling CI, Yuen MMS, Boone CK, Cooke JEK, Bohlmann J, Huber DPW, Murray BW, Coltman DW, Sperling FAH. 2014. How the mountain pine beetle (Dendroctonus ponderosae) breached the Canadian Rocky Mountains. Molecular Biology and Evolution 31 (7), 1803-1815. doi: 10.1093/molbev/msu135.

Sena JS, Giguère I, Boyle B, Rigault P, Birol I, Zuccolo A, Ritland K, Ritland C, Bohlmann J, Jones S, Bousquet J, Mackay J. 2014. Evolution of gene structure in the conifer Picea glauca: a comparative analysis of the impact of intron size. BMC Plant Biology 14 (1), 95. doi: 10.1186/1471-2229-14-95.

Alamouti SM, Haridas S, Feau N, Robertson G, Bohlmann J, Breuil C. 2014. Comparative genomics of the pine pathogens and beetle symbionts in the genus Grosmannia. Molecular biology and evolution 31 (6), 1454-1474. doi: 10.1093/molbev/msu102.

Kolosova N, Breuil C, Bohlmann J. 2014. Cloning and characterization of chitinases from interior spruce and lodgepole pine. Phytochemistry 101, 32-39. doi: 10.1016/j.phytochem.2014.02.006.

Hammerbacher A, Paetz C, Wright LP, Fischer TC, Bohlmann J, Davis AJ, Fenning TM, Gershenzon J, Schmidt A. 2014. Flavan-3-ols in Norway spruce: Biosynthesis, accumulation and function in response to attack by the bark beetle-associated fungus Ceratocystis polonica. Plant Physiology 164 (4), 2107-2122. doi: 10.1104/pp.113.232389.

Pateraki I, Andersen-Ranberg J, Hamberger B, Heskes AM, Martens HJ, Zerbe P, Spanner Bach S, Lindberg Møller B, Bohlmann J, Hamberger B. 2014. Manoyl oxide (13R), the biosynthetic precursor of forskolin, is synthesized in specialized root cork cells in Coleus forskohlii. Plant Physiology 164 (3), 1222-1236. doi: 10.1104/pp.113.228429.

2013

Novak M, Krajnc AU, Lah L, Zupanec N, Krasevec N, Krizman M, Bohlmann J, Komel R. 2013. Low-density Ceratocystis polonica inoculation of Norway spruce (Picea abies) triggers accumulation of monoterpenes with antifungal properties. European Journal of Forest Research 1-11. doi: 10.1007/s10342-013-0772-4.

Robert JA, Pitt C, Bonnett TR, Yuen MMS, Keeling CI, Bohlmann J, Huber DPW. 2013. Disentangling detoxification: gene expression analysis of feeding mountain pine beetle illuminates molecular-level host chemical defense detoxification mechanisms. PloS One 8 (11). doi: 10.1371/journal.pone.0077777.

Kadow D, Bohlmann J, Philips Mora W, Rohn S, Lieberei R. 2013. Identification of two genotypes of cacao tree. Deutsche Lebensmittel-Rundschau 109 (10), 515-519.

Keeling CI, Chiu CC, Aw T, Li M, Henderson H, Tittiger C, Weng H-B, Blomquist GJ, Bohlmann J. 2013. Frontalin pheromone biosynthesis in the mountain pine beetle, Dendroctonus ponderosae, and the role of isoprenyl diphosphate synthases. Proceedings of the National Academy of Sciences 110 (47), 18838-18843. doi: .

Keeling CI, Henderson H, Li M, Dullat HK, Ohnishi T, Bohlmann J. 2013. CYP345E2, an antenna-specific cytochrome P450 from the mountain pine beetle, Dendroctonus ponderosae Hopkins, catalyses the oxidation of pine host monoterpene volatiles. Insect biochemistry and molecular biology. 43 (12), 1142-1151. doi: 10.1016/j.ibmb.2013.10.001.

Diaz-Chavez ML, Moniodis J, Madilao LL, Jancsik S, Keeling CI, Barbour EL, Ghisalberti EL, Plummer JA, Jones CG, Bohlmann J. 2013. Biosynthesis of sandalwood oil: Santalum album CYP76F cytochromes P450 produce santalols and bergamotol. PLOS ONE 8 (9), e75053. doi: 10.1371/journal.pone.0075053.

Kadow D, Bohlmann J, Philips W, Lieberei R. 2013. Identification of main fine flavour components in two genotypes of the chocolate tree (Theobroma cacao L.). Journal of Applied Botany and Food Quality 86 (1). doi: 10.5073/JABFQ.2013.086.013. 

Boone CK, Keefover-Ring K, Mapes AC, Adams AS, Bohlmann J, Raffa KF. 2013. Bacteria associated with a tree-killing insect reduce concentrations of plant defense compounds. Journal of Chemical Ecology 39 (7), 1003-1006. doi: 10.1007/s10886-013-0313-0.

Birol I, Raymond A, Jackman SD, Pleasance S, Coope R, Taylor GA, Yuen MM, Keeling CI, Brand D, Vandervalk BP, Kirk H, Pandoh P, Moore RA, Zhao Y, Mungall AJ, Jaquish B, Yanchuk A, Ritland C, Boyle B, Bousquet J, Ritland K, Mackay J, Bohlmann J, Jones SJ. 2013. Assembling the 20 Gb white spruce (Picea glauca) genome from whole-genome shotgun sequencing data. Bioinformatics 29 (12), 1492-1497. doi: 10.1093/bioinformatics/btt178.

Haridas S, Wang Y, Lim L, Alamouti SM, Jackman S, Docking R, Robertson G, Birol I, Bohlmann J, Breuil C. 2013. The Genome and Transcriptome of the pine saprophyte Ophiostoma Piceae, and a comparison with the bark beetle-associated pine pathogen Grosmannia clavigera. BMC Genomics 14, 373. doi:10.1186/1471-2164-14-373.

Hammerbacher A, Schmidt A, Wadke N, Wright LP, Schneider B, Bohlmann J, Brand WA, Fenning TM, Gershenzon J, Paetz C. 2013. A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce. Plant Physiology 162 (3), 1324-1336.

Zerbe P, Hamberger B, Yuen MM, Chiang A, Sandhu HK, Madilao LL, Nguyen A, Hamberger B, Bach SS, Bohlmann J. 2013. Gene Discovery of Modular Diterpene Metabolism in Nonmodel Systems. Plant Physiology 162 (2), 1073-1091. doi: 10.1104/pp.113.218347.

Nystedt B, Street NR, Wetterbom A, Zuccolo A, Lin YC, Scofield DG, Vezzi F, Delhomme N, Giacomello S, Alexeyenko A, Vicedomini R, Sahlin K, Sherwood E, Elfstrand M, Gramzow L, Holmberg K, Hällman J, Keech O, Klasson L, Koriabine M, Kucukoglu M, Käller M, Luthman J, Lysholm F, Niittylä T, Olson A, Rilakovic N, Ritland C, Rosselló JA, Sena J, Svensson T, Talavera-López C, Theißen G, Tuominen H, Vanneste K, Wu ZQ, Zhang B, Zerbe P, Arvestad L, Bhalerao R, Bohlmann J, Bousquet J, Garcia Gil R, Hvidsten TR, de Jong P, MacKay J, Morgante M, Ritland K, Sundberg B, Thompson SL, Van de Peer Y, Andersson B, Nilsson O, Ingvarsson PK, Lundeberg J, Jansson S. 2013. The Norway spruce genome sequence and conifer genome evolution. Nature 497, 579-584. doi: 10.1038/nature12211.

Hall DE, Yuen MMS, Jancsik S, Lara Quesada A, Dullat HK, Li M, Henderson H, Arango-Velez A, Liao NY, Docking TR, Chan SK, Cooke JEK, Breuil C, Keeling CI, Bohlmann J. 2013. Transcriptome resources and functional characterization of monoterpene synthases for two host species of the mountain pine beetle, lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana).  BMC Plant Biology 13:80. doi:10.1186/1471-2229-13-80.

Xiao M, Zhang Y, Chen X, Lee EJ, Barber CJ, Chakrabarty R, Desgagné-Penix I, Haslam TM, Kim YB, Liu E, Macnevin G, Masada-Atsumi S, Reed DW, Stout JM, Zerbe P, Zhang Y, Bohlmann J, Covello PS, De Luca V, Page JE, Ro DK, Martin VJ, Facchini PJ, Sensen CW. 2013. Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest. Journal of Biotechnology 166 (3), 122-134. doi: 10.1016/j.jbiotec.2013.04.004.

Foster AJ, Hall DE, Mortimer L, Abercromby S, Gries R, Gries G, Bohlmann J, Russell J, Mattsson J. 2013. Identification of Genes in Thuja plicata Foliar Terpenoid Defenses. Plant Physiology 161(4), 1993-2004. doi: 10.1104/pp.112.206383.

Andersson MN, Grosse-Wilde E, Keeling CI, Bengtsson JM, Yuen MM, Li M, Hillbur Y, Bohlmann J, Hansson BS, Schlyter F. 2013. Antennal transcriptome analysis of the chemosensory gene families in the tree killing bark beetles, Ips typographus and Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae). BMC Genomics, 14. doi:10.1186/1471-2164-14-198. 

Janocha S, Zapp J, Hutter M, Kleser M, Bohlmann J, Bernhardt R. 2013. Resin acid conversion with CYP105A1: an enzyme with potential for the production of pharmaceutically relevant diterpenoids. ChemBioChem 14(4), 467-473. doi: 10.1002/cbic.201200729.

Keeling CI, Yuen MMS, Liao NY, Docking TR, Chan SK, Taylor GA, Palmquist DL, Jackman SD, Nguyen A, Li M, Henderson H, Janes JK, Zhao Y, Pandoh P, Moore R, Sperling FAH, Huber DPW, Birol I, Jones SJM, Bohlmann J. 2013. Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest. Genome Biology 14, R27 doi: 10.1186/gb-2013-14-3-r27. 

Tsui CK, DiGuistini S, Feau N, Wang Y, Bohlmann J, Hamelin RC. 2013. Unequal recombination and evolution of the mating-type (MAT) loci in the pathogenic fungus Grosmannia clavigera and relatives. G3: Genes, Genomes, Genetics 3 (3), 465-480. doi: 10.1534/g3.112.004986.

Boone CK, Keefover-Ring K, Mapes AC, Adams AS, Bohlmann J, Raffa KF. 2013. Bacteria Associated with a Tree-Killing Insect Reduce Concentrations of Plant Defense Compounds. Journal of Chemical Ecology 39 (7), 1003-1006. doi: 10.1007/s10886-013-0313-0.
 
Reid K, Holliday J, Yuen M, Nguyen A, Aitken S, Bohlmann J. 2013. Sequencing of Sitka spruce (Picea sitchensis) cDNA libraries constructed from autumn buds and foliage reveals autumn-specific spruce transcripts. Tree Genetics & Genomes 1-9. doi: 10.1007/s11295-012-0584-6.


Hall DE, Zerbe P, Jancsik S, Lara Quesada A, Dullat H, Madilao LL, Yuen M, Bohlmann J. 2013. Evolution of Conifer Diterpene Synthases: Diterpene Resin Acid Biosynthesis in Lodgepole Pine and Jack Pine Involves Monofunctional and Bifunctional Diterpene Synthases. Plant Physiology 161 (2), 600-616. doi: 10.1104/pp.112.208546.

Wang Y, Lim L, DiGuistini S, Robertson G, Bohlmann J, Breuil C. 2013. A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees. New Phytologist 197 (3), 886-89. doi: 10.1111/nph.12063.

Lah L, Haridas S, Bohlmann J, Breuil C. 2013. The cytochromes P450 of Grosmannia clavigera: Genome organization, phylogeny, and expression in response to pine host chemicals. Fungal Genetics and Biology 50, 72-81. doi: 10.1016/j.fgb.2012.10.002. 

2012

Bonnett TR, Robert JA, Pitt C, Fraser JD, Keeling CI, Bohlmann J, Huber DPW. 2012. Global and comparative proteomic profiling of overwintering and developing mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae) larvae. Insect Biochemistry and Molecular Biology 42 (12) :890-901. doi:10.1016/j.ibmb.2012.08.003.

Keeling CI, Henderson H, Li M, Yuen M, Clark EL, Fraser JD, Huber DPW, Liao NY, Docking TR, Birol I, Chan SK, Taylor GA, Palmquist D, Jones SJM, Bohlmann J. 2012. Transcriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests. Insect Biochemistry and Molecular Biology 42 (8) :525-536 doi: 10.1016/j.ibmb.2012.03.

Martin DM, Chiang A, Lund ST, Bohlmann J. 2012. Biosynthesis of wine aroma: transcript profiles of hydroxymethylbutenyl diphosphate reductase, geranyl diphosphate synthase, and linalool/nerolidol synthase parallel monoterpenol glycoside accumulation in Gewürztraminer grapes. Planta :doi: 10.1007/s004250121704-0.

Khadempour L, Lemay V, Jack D, Bohlmann J, Breuil C. 2012. The relative abundance of mountain pine beetle fungal associates changes through the beetle life cycle in pine trees. Microbial Ecology :doi: 10.1007/s00248-012-0077-z.

Bohlmann J. 2012. Pine terpenoid defences in the mountain pine beetle epidemic and in other conifer pest interactions: specialized enemies are eating holes into a diverse, dynamic and durable defence system. Tree Physiology 32 (8) :943-945. doi: 10.1093/treephys/tps065.

Caniard A, Zerbe P, Legrand S, Cohade A, Valot N, Magnard J-L, Bohlmann J, Legendre L. 2012. Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture. BMC Plant Biology 12 :119 doi:10.1186/1471-2229-12-119.

Kelleher CT, Wilkin J, Zhuang J, Cortés AJ, Pérez Quintero AL, Gallagher TF, Bohlmann J, Douglas CJ, Ellis BE, and K. Ritland. 2012. SNP discovery, gene diversity, and linkage disequilibrium in wild populations of Populus tremuloides. Tree Genetics and Genomes 8 (4) :821-829. doi: 10.1007/s11295-012-0467-x.

Tsui CKM, Amanda R, El-Kassaby Y, Rice A, Alamouti SM, Sperling F, Cooke J, Bohlmann J, and R Hamelin. 2012. Population structure and migration pattern of a conifer pathogen, Grosmannia clavigera, as influenced by its symbiont, the mountain pine beetle. Molecular Ecology 21 :71–86.

Buschiazzo E, Ritland C, Bohlmann J, and K Ritland. 2012. Slow but not low: genomic comparisons reveals slower evolutionary rate and higher dN/dS in conifers compared to angiosperms. BMC Evolutionary Biology 12:8.

Facchini PJ, Bohlmann J, Covello PS, De Luca V, Mahadevan R, Page JE, Ro D-K, Sensen CW, Storms R, and VJJ Martin. 2012. Synthetic biosystems for the production of high-value plant metabolites. Trends in Biotechnology (featured on the April 2012 cover of Trends in Biotechnology) 30 :127-131.

Zerbe P, Chiang A, Yuen M, Hamberger B, Hamberger Br, Draper JA, Britton R, and Bohlmann J. 2012. Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production. Journal of Biological Chemistry 287 :12121–12131.

 

Kolosova N and Bohlmann J. 2012. Conifer defense against insects and fungal pathogens. Submitted for publication in: "Growth and Defence in Plants: Resource Allocation at Multiple Scales, Editor R. Matyssek, Springer.


Zerbe P, Chiang A, Bohlmann J. 2012. Mutational analysis of white spruce (Picea glauca) ent-kaurene synthase (PgKS) reveals common and distinct mechanisms of conifer diterpene synthases of general and specialized metabolism. Phytochemistry 74:30-9. doi: 10.1016/j.phytochem.2011.11.004.

2011

Tsui CK, Roe AD, El-Kassaby YA, Rice AV, Alamouti SM, Sperling FA, Cooke JE, Bohlmann J, Hamelin RC. 2011. Population structure and migration pattern of a conifer pathogen, Grosmannia clavigera, as influenced by its symbiont, the mountain pine beetle. Mol Ecol. 21 (1) :71-86.

Bohlmann J. 2011. Terpenoid synthases-from chemical ecology and forest fires to biofuels and bioproducts. Structure 19 (12) :1730-1.

Keeling CI, Weißhaar S, Ralph SG, Jancsik S, Hamberger Br, Dullat HK, and Bohlmann J. 2011. Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp.). BMC Plant Biology 11:43.

Diguistini S, Wang Y, Liao N, Taylor G, Tanguay P, Feau N, Henrissat B, Chan S, Hesse-Orce U, Massoumi Alamouti S, Tsui CKM, Docking R, Levasseur A, Robertson G, Birol I, Holt R, Marra M, Hirst M, Hamelin R, Jones S, Bohlmann J*, and C Breuil. 2011. Genome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen. Proceedings of the National Academy of Sciences 108 (6) :2504-9.

Hall DE, Robert JA, Keeling CI, Domanski D, Quesada AL, Jancsik S, Kuzyk M, Hamberger Br, Borchers CH, and Bohlmann J. 2011. An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil. The Plant Journal 65 (6) :936-48. 

Chen F, Tholl D, Bohlmann J, and E Pichersky. 2011. The family of terpene synthases in plants: A mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom. The Plant Journal 66 (1) :212-29.

Antunes LCM, Arena ET, Menendez A, Han J, Ferreira RBR, Buckner MCC, Loli P, Madilao LL, Bohlmann J, Borchers CH and BB Finlay. 2011. The impact of Salmonella infection on host hormone metabolism revealed by metabolomics. Infection and Immunity 79 (4) :1759-69.

Stephenson PG, Harris N, Cottrell JE, Ralph SG, Bohlmann J, and G Taylor. 2011. A transcriptomic approach to identify genes associated with wood density in Picea sitchensis. Scandinavian Journal of Forest Research 26 :82-96.

Samarasekera N, Keeling CI, Bohlmann J, and B Murray. 2011. Isolation and characterization of EST-derived microsatellite markers for the mountain pine beetle (Dendroctonus ponderosae Hopkins). Molecular Ecology Resources 11 :586-589.

Jones CG, Moniodis J, Zulak KG, Scaffidi A, Plummer JA, Ghisalberti EL, Barbour EL, and Bohlmann J. 2011. Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both the TPS-a and TPS-b subfamilies, including santalene synthases. Journal of Biological Chemistry 286 (20) :17445-54.

Keeling CI, Madilao LL, Zerbe P, Dullat HK, and Bohlmann J. 2011. The primary diterpene synthase products of Picea abies levopimaradiene/abietadiene synthase (PaLAS) are epimers of a thermally unstable diterpenol. Journal of Biological Chemistry 286 (24) :21145-53. (2011) 

Boone CK, Aukema BH, Bohlmann J, Carroll AL, and KF. 2011. Efficacy of tree defense physiology varies with bark beetle population density: a basis for positive feedback in eruptive species. Canadian Journal of Forest Research 41 :1174-1188.

Alamouti SM, Wang V, DiGuistini S, Six DL, Bohlmann J, Hamelin RC, Feau N, and C Breuil. 2011. Gene genealogies reveal cryptic species and host preference for the pine fungal pathogen Grosmannia clavigera. Molecular Ecology 20 (12) :2581-602.

Haridas S, Breuill C, Bohlmann J, and T Hsiang. 2011. A biologist's guide to de novo genome assembly using next-generation sequence data: A test with fungal genomes. Journal of Microbiological Methods 86 (3) :368-75.

Adams A, Boone CK, Bohlmann J and K Raffa. 2011. Responses of bark beetle-associated bacteria to host monoterpenes and their relationship to insect life histories. Journal of Chemical Ecology 37 (8) :808–817.

Lah L, Podobnik B, Novak M, Korošec B, Berne S, Vogelsang M, Kraševec N, Zupanec N, Stojan J, Bohlmann J, and R Komel. 2011.  The versatility of the fungal cytochrome P450 monooxygenase system is instrumental in xenobiotic detoxification.  Molecular Microbiology 81 (5) :1374-1389.


Hammerbacher A, Ralph SG, Bohlmann J, Fenning TM, Gershenzon J, and A Schmidt. 2011. Biosynthesis of the major tetrahydroxystilbenes in spruce, astringin and isorhapontin, proceeds via resveratrol and is enhanced by fungal infection. Plant Physiology 157 (2) :876-90.

Bleeker P, Spyropoulou E, Diergaarde P, Volpin H, De Both MTJ, Zerbe P, Bohlmann J, Falara V, Matsuba Y, Pichersky E, Haring M, and R Schuurink. 2011. RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes. Plant Molecular Biology 77 (4) :323-36. )

Hamberger B, Ohnishi T, Hamberger B, Séguin A, and Bohlmann J. 2011. Evolution of diterpene metabolism: Sitka spruce CYP720B4 catalyses multiple oxidations in resin acid biosynthesis of conifer defense against insects. Plant Physiology 157(4):1677-95.

2010

Zulak KG, Dullat HK, Keeling CI, Lippert D, and Bohlmann J. Immunofluorescence localization of levopimaradiene/abietadiene synthase in methyl jasmonate treated stems of Sitka spruce (Picea sitchensis) shows activation of diterpenoid biosynthesis in cortical and developing traumatic resin ducts. Phytochemistry 71 (14) :1695-9. (2010) 

Philippe RN, Ralph SG, Mansfield SD, and Bohlmann J. Transcriptome profiles of hybrid poplar (Populus trichocarpa x deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria). New Phytologist 71 (14) :1695-9. (2010) 

Robert JA and Bohlmann J. Behavioral and reproductive response of white pine weevil (Pissodes strobi) to resistant and susceptible Sitka spruce (Picea sitchensis). Insects 1 :3-19. (2010) 

Robert JA, Madilao LL, White R. Yanchuk A, King J, and Bohlmann J. Terpenoid metabolite profiling in Sitka spruce identifies association of dehydroabietic acid, (+)-3-carene and terpinolene with resistance against white pine weevil. Botany 65 (6) :936-48. (2010) 

Tsui CKM, Wang B, Khadempour L, Alamouti SM, Bohlmann J, Murray BW, and RC Hamelin. Rapid identification and detection of pine pathogenic fungi associated with mountain pine beetles by padlock probes. Journal of Microbiological Methods 83 (1) :26-33. (2010)

Khadempour L, Massoumi Alamouti S, Hamelin R, Bohlmann J,and C Breuil. Target-specific PCR primers can detect and differentiate ophiostomatoid fungi from microbial communities associated with the mountain pine beetle Dendroctonus ponderosae. Fungal Biology 14 (10) :825-33. (2010)

Hesse-Orce U, Diguistini S, Keeling CI, Wang Y, Docking R, Liao NY, Robertson G, Holt RA, Jones SJM, Bohlmann J, and C Breuil. Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera. BMC Genomics 11 :536. (2010)

Martin DM, Aubourg S, Schouwey MB, Daviet L, Schalk M, Toub O, Lund ST, and Bohlmann J. Functional annotation, genome organization and phylogeny of the grapevine (Vitis vinifera) terpene synthase gene family based on genome assembly, FLcDNA cloning, and enzyme assays. BMC Plant Biology 10 :226. (2010) 

Zulak KG and Bohlmann J. Terpenoid biosynthesis and specialized vascular cells of conifer defense. Journal of Integrative Plant Biology 52 :86 – 97 (DOI: 10.1111/j.1744-7909.2010.00910.x). (2010) 

Keeling CI, Dullat HK, Yuen M, Ralph SG, Jancsik S, and Bohlmann J. Identification and functional characterization of monofunctional ent-copalyl diphosphate and ent-kaurene synthases in white spruce (Picea glauca) reveal different patterns for diterpene synthase evolution for primary and secondary metabolism in gymnosperm. Plant Physiology 152 :1197–1208 (DOI:10.1104/pp.109.151456). (2010) 

Abbott E, Hall D, Hamberger B, and Bohlmann J. Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca). BMC Plant Biology 10 (106) :(DOI:10.1186/1471-2229-10-106). (2010) 

Wang Y, DiGuistini S, Wang T-CT, Bohlmann J, and C Breuil. Agrobacterium-meditated gene disruption using split-marker in Grosmannia clavigera, a mountain pine beetle associated pathogen. Current Genetics 56 :297-307 (DOI 10.1007/s00294-010-0294-2). (2010) 

Albouyeh R, Farzaneh N, Bohlmann J, and K Ritland. Multivariate analysis of digital gene expression profiles identifies a xylem signature of the vascular tissue of white spruce (Picea glauca). Tree Genetics & Genomes". Tree Genetics & Genomes :6: 601-611 (DOI 10.1007/s11295-010-0275-0). (2010)

2009

Philippe RN, Ralph SG, Külheim C, Jancsik SI, and Bohlmann J. Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family. New Phytologist 184 (4) :865-84. (2009) 

Tsui CKM, Feau N, Ritland CE, Massoumi Alamouti S, DiGuistini S, Bohlmann J, Breuil C, and RC Hamelin. Characterization of microsatellite loci in the fungus Grosmannia clavigera, a pine pathogen associated with the mountain pine beetle. Molecular Ecology Resources 9 :1500-1503 (DOI: 10.1111/j1755-0998.2009.02717.x). (2009)

Lippert DN, Ralph SG, Phillips M, White R, Smith D, Hardie D, Gershenzon J, Ritland K, Borchers CH, Bohlmann J. Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response. Proteomics 9 (2) :350-67. (2009)

Lippert D, Yuen M, and Bohlmann J. Spruce Proteome DB: A resource for conifer proteomics research. Tree Genetics & Genomes (5) :723 – 727. (2009) 

Diguistini S, Liao NY, Platt D, Robertson G, Seidel M, Chan SK, Docking TR, Birol I, Holt RA, Hirst M, Mardis E, Marra MA, Hamelin RC, Bohlmann J, Breuil C, Jones SJ. De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data. Genome Biol 10 (9) :R94. (2009)

Hamberger B, Hall D, Yuen M, Oddy C, Hamberger B, Keeling CI, Ritland C, Ritland K, Bohlmann J. Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome. BMC Plant Biol 9106 (2009)

Bohlmann J, Gershenzon J. Old substrates for new enzymes of terpenoid biosynthesis. Proc Natl Acad Sci U S A 106 (26) :10402-3. (2009)
Dafoe NJ, Zamani A, Ekramoddoullah AK, Lippert D, Bohlmann J, Constabel CP. "Analysis of the poplar phloem proteome and its response to leaf wounding". J Proteome Res 8 (5) :2341-50. (2009)

Martin DM, Toub O, Chiang A, Lo BC, Ohse S, Lund ST, Bohlmann J. The bouquet of grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) flowers arises from the biosynthesis of sesquiterpene volatiles in pollen grains. Proc Natl Acad Sci U S A 106 (17) :7245-50. (2009)

Liewlaksaneeyanawin C, Zhuang J, Tang M, Farzaneh N, Lueng G, Oddy C, Findlay S, Ritland CE, Bohlmann J, and K Ritland. Identification and validation of COS markers in Pinaceae. Tree Genetics & Genomes (5) :247-255. (2009)

Zulak KG, Lippert DN, Kuzyk MA, Domanski D, Chou T, Borchers CH, Bohlmann J. Targeted proteomics using selected reaction monitoring (SRM) reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate treated Norway spruce (Picea abies). Plant J (2009)

2008

Godard KA, White R, Bohlmann J. Monoterpene-induced molecular responses in Arabidopsis thaliana. Phytochemistry 69 (9) :1838-49. (2008) 

Bohlmann J, Keeling CI. Terpenoid biomaterials. Plant J 54 (4) :656-69. (2008)

Ehlting J, Chowrira SG, Mattheus N, Aeschliman DS, Arimura G, Bohlmann J. Comparative transcriptome analysis of Arabidopsis thaliana infested by diamond back moth (Plutella xylostella) larvae reveals signatures of stress response, secondary metabolism, and signalling. BMC Genomics 9 :154. (2008)

Ralph SG, Chun HJ, Cooper D, Kirkpatrick R, Kolosova N, Gunter L, Tuskan GA, Douglas CJ, Holt RA, Jones SJ, Marra MA, Bohlmann J. Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feeding. BMC Genomics 9 :57. (2008)

Keeling CI, Weisshaar S, Lin RP, Bohlmann J. Functional plasticity of paralogous diterpene synthases involved in conifer defense. Proc Natl Acad Sci U S A 105 (3) :1085-90. (2008)

Holliday JA, Ralph SG, White R, Bohlmann J, Aitken SN. Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis). New Phytol 178 (1) :103-22. (2008)

Bohlmann J. Insect-induced terpenoid defenses in spruce. ln: "Induced Plant Resistance to Herbivory" A. Schaller (ed.)". Springer Science :137-187. (2008)

Keeling CI and Bohlmann J. Terpenoids in plants. Wiley Encyclopedia of Chemical Biology, doi: 10.1002/9780470048672.webcb596 (2008)

Souza Cde A, Barbazuk B, Ralph SG, Bohlmann J, Hamberger B, Douglas CJ. Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella. New Phytol 179 (4) :987-1003. (2008)

Jones CG, Keeling CI, Ghisalberti EL, Barbour EL, Plummer JA, Bohlmann J. Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L. Arch Biochem Biophys 477 (1) :121-30. (2008)

Ralph SG, Chun HJ, Kolosova N, Cooper D, Oddy C, Ritland CE, Kirkpatrick R, Moore R, Barber S, Holt RA, Jones SJ, Marra MA, Douglas CJ, Ritland K, Bohlmann J. A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis). BMC Genomics 9 (484) (2008)

2007

Godard KA, Byun-McKay A, Levasseur C, Plant A, Seguin A, Bohlmann J. Testing of a heterologous, wound- and insect-inducible promoter for functional genomics studies in conifer defense. Plant Cell Rep 26 (12) :2083-90. (2007) 

Peng FY, Reid KE, Liao N, Schlosser J, Lijavetzky D, Holt R, Martínez Zapater JM, Jones S, Marra M, Bohlmann J, Lund ST. Generation of ESTs in Vitis vinifera wine grape (Cabernet Sauvignon) and table grape (Muscat Hamburg) and discovery of new candidate genes with potential roles in berry development. Gene 402 (1) :40-50. (2007)

Phillips MA, Walter MH, Ralph SG, Dabrowska P, Luck K, Urós EM, Boland W, Strack D, Rodríguez-Concepción M, Bohlmann J, Gershenzon J. Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies). Plant Mol Biol 65 (3) :243-57. (2007)

Behnke K, Ehlting B, Teuber M, Bauerfeind M, Louis S, Hänsch R, Polle A, Bohlmann J, Schnitzler JP. Transgenic, non-isoprene emitting poplars don't like it hot. Plant J 51 (3) :485-99. (2007)

Ralph SG, Jancsik S, Bohlmann J. Dirigent proteins in conifer defense II: Extended gene discovery, phylogeny, and constitutive and stress-induced gene expression in spruce (Picea spp.). Phytochemistry 68 (14) :1975-91. (2007)

Miranda M, Ralph SG, Mellway R, White R, Heath MC, Bohlmann J, Constabel CP. The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to infection by Melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins. Mol Plant Microbe Interact 20 (7) :816-31. (2007)

Kelleher CT, Chiu R, Shin H, Bosdet IE, Krzywinski MI, Fjell CD, Wilkin J, Yin T, DiFazio SP, Ali J, Asano JK, Chan S, Cloutier A, Girn N, Leach S, Lee D, Mathewson CA, Olson T, O'connor K, Prabhu AL, Smailus DE, Stott JM, Tsai M, Wye NH, Yang GS, Zhuang J, Holt RA, Putnam NH, Vrebalov J, Giovannoni JJ, Grimwood J, Schmutz J, Rokhsar D, Jones SJ, Marra MA, Tuskan GA, Bohlmann J, Ellis BE, Ritland K, Douglas CJ, Schein JE. A physical map of the highly heterozygous Populus genome: integration with the genome sequence and genetic map and analysis of haplotype variation. Plant J 50 (6) :1063-78. (2007)

Huber DP, Erickson ML, Leutenegger CM, Bohlmann J, Seybold SJ. Isolation and extreme sex-specific expression of cytochrome P450 genes in the bark beetle, Ips paraconfusus, following feeding on the phloem of host ponderosa pine, Pinus ponderosa. Insect Mol Biol 16 (3) :335-49. (2007)

DiGuistini S, Ralph SG, Lim YW, Holt R, Jones S, Bohlmann J, Breuil C. Generation and annotation of lodgepole pine and oleoresin-induced expressed sequences from the blue-stain fungus Ophiostoma clavigerum, a Mountain Pine Beetle-associated pathogen. FEMS Microbiol Lett 267 (2) :151-8. (2007)

Friedmann M, Ralph SG, Aeschliman D, Zhuang J, Ritland K, Ellis BE, Bohlmann J, Douglas CJ. Microarray gene expression profiling of developmental transitions in Sitka spruce (Picea sitchensis) apical shoots. J Exp Bot 58 (3) :593-614. (2007)

Lippert D, Chowrira S, Ralph SG, Zhuang J, Aeschliman D, Ritland C, Ritland K, Bohlmann J. Conifer defense against insects: proteome analysis of Sitka spruce (Picea sitchensis) bark induced by mechanical wounding or feeding by white pine weevils (Pissodes strobi). Proteomics 7 (2) :248-70. (2007)

Ralph SG, Hudgins JW, Jancsik S, Franceschi VR, Bohlmann J. Aminocyclopropane carboxylic acid synthase is a regulated step in ethylene-dependent induced conifer defense. Full-length cDNA cloning of a multigene family, differential constitutive, and wound- and insect-induced expression...". Plant Physiol 143 (1) :410-24. (2007)

Berube Y, Zhuang J, Ralph S, Rungis D, Bohlmann J, and K Ritland. Characterization of EST-SSRs in loblolly pine and spruce. Tree Genetics & Genomics 3 :251-259. (2007)

Philippe R.N. and Bohlmann J. Poplar defense against insect herbivores. Canadian Journal of Botany 85 :111-1126. (2007)

2006

Hamberger B, Bohlmann J. Cytochrome P450 mono-oxygenases in conifer genomes: discovery of members of the terpenoid oxygenase superfamily in spruce and pine. Biochem Soc Trans 34 (6) :1209-14. (2006) 

Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313 (5793) :1596-604. (2006)

Hudgins JW, Ralph SG, Franceschi VR, and Bohlmann J. Ethylene in induced conifer defense response: FLcDNA cloning, wound-induced protein expression, and cellular and subcellular localization of 1-aminocyclopropane-carboxylic acid oxidase (ACO) in specialized resin duct cells and polyphenolic phloem parechny. Planta (224) :865-877. (2006) 

Seybold SJ, Huber DPW, Lee LC, and Bohlmann J. Pine monoterpenes and pine bark beetles: A marriage of convenience for defense and chemical communication. Phytochemistry Reviews (5) :143-178. (2006) 

Phillips M, Bohlmann J, and J Gershenzon. Molecular regulation of induced terpenoid biosynthesis in conifers. Phytochemistry Reviews (5) :179-189. (2006)

Ralph SG, Yueh H, Friedmann M, Aeschliman D, Zeznik JA, Nelson CC, Butterfield YS, Kirkpatrick R, Liu J, Jones SJ, Marra MA, Douglas CJ, Ritland K, Bohlmann J. Conifer defence against insects: microarray gene expression profiling of Sitka spruce (Picea sitchensis) induced by mechanical wounding or feeding by spruce budworms (Choristoneura occidentalis) or white pine weevils (Pissodes strobi) reveals large-scale changes of the host transcriptome. Plant Cell Environ 29 (8) :1545-70. (2006)

Ro DK, Bohlmann J. Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): Functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1). Phytochemistry 67 (15) :1572-8. (2006)

Ro DK, Ehlting J, Keeling CI, Lin R, Mattheus N, Bohlmann J. Microarray expression profiling and functional characterization of AtTPS genes: duplicated Arabidopsis thaliana sesquiterpene synthase genes At4g13280 and At4g13300 encode root-specific and wound-inducible (Z)-gamma-bisabolene synthases. Arch Biochem Biophys 448 (1) :104-16. (2006)

Ralph S, Oddy C, Cooper D, Yueh H, Jancsik S, Kolosova N, Philippe RN, Aeschliman D, White R, Huber D, Ritland CE, Benoit F, Rigby T, Nantel A, Butterfield YS, Kirkpatrick R, Chun E, Liu J, Palmquist D, Wynhoven B, Stott J, Yang G, Barber S, Holt RA, Siddiqui A, Jones SJ, Marra MA, Ellis BE, Douglas CJ, Ritland K, Bohlmann J. Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences. Mol Ecol 15 (5) :1275-97. (2006)

Gilchrist EJ, Haughn GW, Ying CC, Otto SP, Zhuang J, Cheung D, Hamberger B, Aboutorabi F, Kalynyak T, Johnson L, Bohlmann J, Ellis BE, Douglas CJ, Cronk QC. Use of Ecotilling as an efficient SNP discovery tool to survey genetic variation in wild populations of Populus trichocarpa. Mol Ecol 15 (5) :1367-78. (2006)

Byun-McKay A, Godard KA, Toudefallah M, Martin DM, Alfaro R, King J, Bohlmann J, Plant AL. Wound-induced terpene synthase gene expression in Sitka spruce that exhibit resistance or susceptibility to attack by the white pine weevil. Plant Physiol 140 (3) :1009-21. (2006)

Lund ST, Bohlmann J. The molecular basis for wine grape quality--a volatile subject. Science 311 (5762) :804-5. (2006)

Ralph S, Park JY, Bohlmann J, Mansfield SD. Dirigent proteins in conifer defense: gene discovery, phylogeny, and differential wound- and insect-induced expression of a family of DIR and DIR-like genes in spruce (Picea spp.). Plant Mol Biol 60 (1) :21-40. (2006)

Keeling CI, Bohlmann J. Genes, enzymes and chemicals of terpenoid diversity in the constitutive and induced defence of conifers against insects and pathogens. New Phytol 170 (4) :657-75. (2006)

Ritland K, Ralph S, Lippert D, Rungis D, and Bohlmann J. A new direction in conifer genomics. In: Landscapes, Genomics and Transgenic Conifer Forests. Claire Williams (ed.) Springer Publishers, Dordrecht Netherlands :75-84. (2006) 

2005

Huber DP, Philippe RN, Madilao LL, Sturrock RN, Bohlmann J. Changes in anatomy and terpene chemistry in roots of Douglas-fir seedlings following treatment with methyl jasmonate. Tree Physiol 25 (8) :1075-83. (2005) 

Ehlting J, Mattheus N, Aeschliman DS, Li E, Hamberger B, Cullis IF, Zhuang J, Kaneda M, Mansfield SD, Samuels L, Ritland K, Ellis BE, Bohlmann J, Douglas CJ. Global transcript profiling of primary stems from Arabidopsis thaliana identifies candidate genes for missing links in lignin biosynthesis and transcriptional regulators of fiber differentiation. Plant J 42 (5) :618-40. (2005)

Huber DP, Philippe RN, Godard KA, Sturrock RN, Bohlmann J. Characterization of four terpene synthase cDNAs from methyl jasmonate-induced Douglas-fir, Pseudotsuga menziesii. Phytochemistry 66 (12) :1427-39. (2005)

Ro DK, Arimura G, Lau SY, Piers E, Bohlmann J. Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase. Proc Natl Acad Sci U S A 102 (22) :8060-5. (2005)

Lippert D, Zhuang J, Ralph S, Ellis DE, Gilbert M, Olafson R, Ritland K, Ellis B, Douglas CJ, Bohlmann J. Proteome analysis of early somatic embryogenesis in Picea glauca. Proteomics 5 (2) :461-73. (2005)

Miller B, Madilao LL, Ralph S, Bohlmann J. Insect-induced conifer defense. White pine weevil and methyl jasmonate induce traumatic resinosis, de novo formed volatile emissions, and accumulation of terpenoid synthase and putative octadecanoid pathway transcripts in Sitka spruce. Plant Physiol 137 (1) :369-82. (2005)

Martin D and Bohlmann J. Molecular biochemistry and genomics of terpenoid defenses in conifers. Recent Advances in Phytochemistry (39) :29-56. (2005)

Rungis D, Hamberger B, Berube Y, Wilkin J, Bohlmann J, and K Ritland. Efficient genetic mapping of single nucleotide polymorphisms based upon DNA mismatch digestion. Molecular Breeding (16) :261-270. (2005)

2004

Huber DP, Ralph S, Bohlmann J. Genomic hardwiring and phenotypic plasticity of terpenoid-based defenses in conifers. J Chem Ecol 30 (12) :2399-418. (2004) 

Rungis D, Berube Y, Zhang J, Ralph S, Ritland CE, Ellis BE, Douglas C, Bohlmann J, Ritland K. Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags. Theor Appl Genet 109 (6) :1283-94. (2004)

Lucker J, Bowen P, Bohlmann J. Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries. Phytochemistry 65 (19) :2649-59. (2004)

Chen F, Ro DK, Petri J, Gershenzon J, Bohlmann J, Pichersky E, Tholl D. Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole. Plant Physiol 135 (4) :1956-66. (2004)

Arimura G, Ozawa R, Kugimiya S, Takabayashi J, Bohlmann J. Herbivore-induced defense response in a model legume. Two-spotted spider mites induce emission of (E)-beta-ocimene and transcript accumulation of (E)-beta-ocimene synthase in Lotus japonicus. Plant Physiol 135 (4) :1976-83. (2004)

Martin DM, Fäldt J, Bohlmann J. Functional characterization of nine Norway Spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily. Plant Physiol 135 (4) :1908-27. (2004)

Reiling KK, Yoshikuni Y, Martin VJ, Newman J, Bohlmann J, Keasling JD. Mono and diterpene production in Escherichia coli. Biotechnol Bioeng 87 (2) :200-12. (2004)

Kolosova N, Miller B, Ralph S, Ellis BE, Douglas C, Ritland K, Bohlmann J. Isolation of high-quality RNA from gymnosperm and angiosperm trees. Biotechniques 36 (5) :821-4. (2004)

Martin DM, Bohlmann J. Identification of Vitis vinifera (-)-alpha-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase. Phytochemistry 65 (9) :1233-9. (2004)

Arimura G, Huber DP, Bohlmann J. Forest tent caterpillars (Malacosoma disstria) induce local and systemic diurnal emissions of terpenoid volatiles in hybrid poplar (Populus trichocarpa x deltoides): cDNA cloning, functional characterization, and patterns of gene expression .... Plant J 37 (4) :603-16. (2004)

Huber DPW and Bohlmann J. Terpene synthases and the mediation of plant-insect ecological interactions by terpenoids: a mini-review. In: Plant Adaptation: Molecular Genetics and Ecology. Q.C.B. Cronck, J. Whitton, R.H. Ree, I.E.P. Taylor". NRC Research Press, Ottawa, Ontario 70 :81. (2004) 

2003

McKay SA, Hunter WL, Godard KA, Wang SX, Martin DM, Bohlmann J, Plant AL. Insect attack and wounding induce traumatic resin duct development and gene expression of (-)-pinene synthase in Sitka spruce. Plant Physiol 133 (1) :368-78. (2003) 

Martin DM, Gershenzon J, Bohlmann J. Induction of volatile terpene biosynthesis and diurnal emission by methyl jasmonate in foliage of Norway spruce. Plant Physiol 132 (3) :1586-99. (2003)

Dudareva N, Martin D, Kish CM, Kolosova N, Gorenstein N, Fäldt J, Miller B, Bohlmann J. (E)-beta-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon: function and expression of three terpene synthase genes of a new terpene synthase subfamily. Plant Cell 15 (5) :1227-41. (2003)

Martin D, Bohlmann J, Gershenzon J, Francke W, Seybold SJ. A novel sex-specific and inducible monoterpene synthase activity associated with a pine bark beetle, the pine engraver, Ips pini. Naturwissenschaften 90 (4) :173-9. (2003)

Fäldt J, Arimura G, Gershenzon J, Takabayashi J, Bohlmann J. Functional identification of AtTPS03 as (E)-beta-ocimene synthase: a monoterpene synthase catalyzing jasmonate- and wound-induced volatile formation in Arabidopsis thaliana. Planta 216 (5) :745-51. (2003)

Fäldt J, Martin D, Miller B, Rawat S, Bohlmann J. Traumatic resin defense in Norway spruce (Picea abies): methyl jasmonate-induced terpene synthase gene expression, and cDNA cloning and functional characterization of (+)-3-carene synthase. Plant Mol Biol 51 (1) :119-33. (2003)

2002

Aubourg S, Lecharny A, Bohlmann J. Genomic analysis of the terpenoid synthase ( AtTPS) gene family of Arabidopsis thaliana. Mol Genet Genomics 267 (6) :730-45. (2002) 

Martin D, Tholl D, Gershenzon J, Bohlmann J. Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant Physiol 129 (3) :1003-18. (2002)

Bohlmann J, Stauber EJ, Krock B, Oldham NJ, Gershenzon J, Baldwin IT. Gene expression of 5-epi-aristolochene synthase and formation of capsidiol in roots of Nicotiana attenuata and N. sylvestris. Phytochemistry 60 (2) :109-16. (2002)

Alfaro RI, Borden JH, King JN, Tomlin ES, McIntosh RL, and Bohlmann J. Mechanisms of Resistance in Conifers against shoot infesting insects. In: Mechanisms and Deployment of Resistance in Trees to Insects. M.R. Wagner, K.M. Clancy, F. Lieutier, and T.D. Paine". Kluwer Academic Press, Dordrecht, the Netherlands :101-126. (2002) 

2000

Bohlmann J, Gershenzon J and S Aubourg. Biochemical, molecular genetic and evolutionary aspects of defense-related terpenoid metabolism in conifers. In: Evolution of metabolic pathways, Romeo J.T. et al. (eds) Recent Advances in Phytochemistry 34, pp. 109 - 150.  (2000)

Bohlmann J, Martin D, Oldham NJ and J Gershenzon. Terpenoid secondary metabolism in Arabidopsis thaliana: cDNA cloning, characterization and functional expression of a myrcene / (E)-b-ocimene synthase. Archives of Biochemistry and Biophysics 375: 261-269.  (2000)

Seybold SJ, Bohlmann J and KF Raffa. The biosynthesis of coniferophagous bark beetle pheromones and conifer isoprenoids: Evolutionary perspective and synthesis. The Canadian Entomologist 132, 697-753.  (2000)

1999

 

Bohlmann J and R Croteau. Diversity and variability of terpenoid defenses in conifers: molecular genetics, biochemistry and evolution of the terpene synthase gene family in grand fir. Novartis Foundation Symposium Series 223, Insect-plant interactions and induced plant defence, pp. 132-149. (1999)

Bohlmann J, Phillips M, Ramachandiran V, Katoh S and R Croteau (1999). cDNA cloning, characterization, and functional expression of four new monoterpene synthases of the TPSd gene family from grand fir (Abies grandis)." Archives of Biochemistry and Biophysics 368, 232-243.  (1999)

1998

Bohlmann J, Crock J, Jetter R and R Croteau. 1998. cDNA cloning, characterization, and functional expression of wound-inducible (E)-a-bisabolene synthase from grand fir (Abies grandis). Proceedings of the National Academy of Sciences USA 95, 6756-6761.

Bohlmann J, Meyer-Gauen G and R Croteau. 1998. Plant terpenoid synthases: molecular biology and phylogenetic analysis. Proceedings of the National Academy of Sciences USA 95, 4126-4133.

Steele CL, Katoh S, Bohlmann J and R Croteau. 1998. Regulation of oleoresinosis in grand fir (Abies grandis): differential transcriptional control of monoterpene, sesquiterpene and diterpene synthase genes in response to wounding." Plant Physiology 116, 1497-1504.

Steele CL, Crock J, Bohlmann J and R Croteau. 1998. Sesquiterpene synthases from gand fir (Abies grandis): comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of d-selinene synthase and g-humulene synthase. Journal of Biological Chemistry 273, 2078-2089.

1997

Bohlmann J, Steele CL and R Croteau. 1997. Monoterpene synthases from grand fir (Abies grandis): cDNA isolation, characterization and functional expression of myrcene synthase, (-)-4S-limonene synthase, and (-)-1S:5S-pinene synthase. Journal of Biological Chemistry 272, 21784-21792.

1996

Bohlmann J, Lins T, Martin W and U Eilert. 1996. Anthranilate synthase from Ruta graveolens: Duplicated ASa genes encode tryptophan-sensitive and tryptophan-insensitive isoenzymes specific to amino acid and alkaloid biosynthesis. Plant Physiology 111, 507-514.

1995

Bohlmann J, DeLuca V, Eilert U and W Martin. 1995. Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: Modes of expression and properties of native and recombinant enzymes. The Plant Journal 7, 491-501.

Bohlmann J, Gibraltarskaya E and U Eilert. 1995. Elicitor induction of furanocoumarin biosynthetic pathway in cell cultures of Ruta graveolens L. Plant Cell Tissue and Organ Culture 43, 155-161.

1994

Bohlmann J and U Eilert. 1994. Elicitor induced secondary metabolism in Ruta graveolens L.: Role of chorismate utilizing enzymes. Plant Cell Tissue and Organ Culture 38, 189-198.

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Joerg Bohlmann, Dezene PW Huber, Brent W Murray, David W Coltman, Felix AH Sperling

Celedon JM, Yuen MMS, Chiang A, Henderson H, Reid KE, and Bohlmann J. Cell-type and tissue specific transcriptomes of the white spruce (Picea glauca) bark unmask fine-scale spatial patterns of constitutive and induced conifer defense. The Plant Journal. doi: 10.1111/tpj.13673.

 

Mageroy MH, Jancsik S, Yuen MMS, Fischer M, Withers SG, P aetz C, Schneider B, and Bohlmann J. A conifer UDP-sugar dependent glycosyltransferase contributes to acetophenone metabolism and defense against insects. Plant Physiology. doi: 10.1104/pp.17.00611

 

Chiu CC, Keeling CI, and Bohlmann J. Toxicity of pine monoterpenes to mountain pine beetle. Scientific Reports. doi: 10.1038/s41598-017-08983-y

 

Mageroy MH, Lachance D, Jancsik S, Parent G, Séguin A, MacKay J, and Bohlmann J. In vivo function of Pgßglu-1 in the release of acetophenones in white spruce. PeerJ. doi: 10.7717/peerj.3535

 

Celedon JM and Bohlmann J. An extended model of heartwood secondary metabolism informed by functional genomics. Tree Physiology. doi: 10.1093/treephys/tpx070

 

Moniodis J, Jones CG, Renton M, Plummer JA, Barbour EL, Ghisalberti EL, and Bohlmann J. Sesquiterpene variation in West Australian Sandalwood (Santalum spicatum). Molecules. doi:10.3390/molecules22060940

 

Burke J, Bohlmann J, and A Carroll. Consequences of distributional asymmetry in a warming environment: invasion of novel forests by the mountain pine beetle. Ecosphere. doi: 10.1002/ecs2.1778

 

Booth JK, Page JE and Bohlmann J. Terpene synthases from Cannabis sativa. PLoS ONE. doi:10.1371/journal.pone.0173911

 

Pavy N, Lamothe M, Pelgas B, Gagnon F, Birol I, Bohlmann J, Mackay J, Isabel N and Bousquet, J. A high resolution reference genetic map positioning 8.8K genes for the conifer white spruce: Structural genomics implications and correspondence with physical distance. Plant J. doi:10.1111/tpj.13478

 

Mafu S, Karunanithi PS, Palazzo TA, Harrod BL, Rodriguez SM, Mollhoff IN, O’Brien TE, Tong S, Fiehn O, Tantillo DJ, Bohlmann J and Zerbe P. Biosynthesis of the microtubule-destabilizing diterpene pseudolaric acid B from golden larch involves an unusual diterpene synthase. PNAS. doi: 10.1073/pnas.1612901114

 

Pelot KA, Mitchell R, Kwon M, Hagelthorn DM, Wardman JF, Chiang A, Bohlmann J, Ro DK and Zerbe P. Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase. Plant J. doi:10.1111/tpj.13427