Publications

Google Scholar

2023

Kruse, Lars, Sunstrum, Federick, Garcia, Daniela, Lopez Perez, Guillermo, Jancsik, Sharon, Bohlmann, Jörg, Irmisch, Sandra. Improved production of the antidiabetic metabolite montbretin A in Nicotiana benthamiana: Discovery, characterization, and use of Crocosmia shikimate shunt genes. The Plant Journal.  In press.

Lo T, Coombe L, Gagalova K, Marr A, Warren R, Kirk H, Pandoh P, Zhao Y, Moore R, Mungall A, Ritland C, Pavy N, Jones S, Bohlmann J, Bousquet J, Birol I, and A Thomson. Assembly and annotation of the black spruce genome provide insights on spruce phylogeny and evolution of stress response. . G3: Genes/Genomes/Genetics (https://doi.org/10.1093/g3journal/jkad247)

Suzana Tiemi Ivamoto-Suzuki, José Miguel Celedón, Macaire M. S. Yuen, Cíntia Sorane Good Kitzberger, Douglas Silva Domingues, Jörg Bohlmann, and Luiz Filipe Protasio Pereira. Functional Characterization of ent-Copalyl Diphosphate Synthase and Kaurene Synthase Genes from Coffea arabica L. ACS Publications (https://doi.org/10.1021/acs.jafc.2c09087)

Tin Hang Hung, Ernest T. Y. Wu, Pauls Zeltiņš, Āris Jansons, Aziz Ullah, Nadir Erbilgin, Joerg Bohlmann, Jean Bousquet, Inanc Birol, Sonya M. Clegg, John J. MacKay. Long-insert sequence capture detects high copy numbers in a defence-related beta-glucosidase gene βglu-1 with large variations in white spruce but not Norway spruce. bioRxiv (doi: https://doi.org/10.1101/2023.08.18.551884)

Whitehill JGA, Bohlmann J, and P Krokene (2023). Forest insect – Plant Interactions.  Chapter 7. In: Forest Entomology and Pathology, Vol. 1. Editors: Jeremy Allison, Timothy D. Paine, Michael J. Wingfield, Bernard Slippers. Springer-Nature.  Wingfield, Bernard Slippers. Springer-Nature.

Janet X. Li, Kleinberg X. Fernandez, Carol Ritland, Sharon Jancsik, Daniel B. Engelhardt, Lauren Coombe, René L. Warren, Marco J. van Belkum, Allan L. Carroll, John C. Vederas, Joerg Bohlmann, Inanc Birol. Genomic virulence features of Beauveria bassiana as a biocontrol agent for the mountain pine beetle population. BMC Genomics (doi.org/10.1186/s12864-023-09473-4)

Justin G. A. Whitehill, Macaire M. S. Yuen, Angela Chiang, Carol E. Ritland, Jörg Bohlmann. Transcriptome features of stone cell development in weevil-resistant and susceptible Sitka spruce. New Phytologist (doi.org/10.1111/nph.19103)

Gwen Huber, Joerg Bohlmann, Kermit Ritland. Variation and Natural Heritability of Blueberry Floral Volatiles. Journal of Agricultural and Food Chemistry. (doi.org/10.1021/acs.jafc.2c06703)

Olivia LuiLharbi DridiEmmanuel GonzalezSuraya YasmineRyszard KubinskiHannah BillingsJoerg BohlmannStephen G. WithersCorinne Maurice, Bastien Castagner. Characterizing the Effect of Amylase Inhibitors on Maltodextrin Metabolism by Gut Bacteria Using Fluorescent Glycan Labeling. ACS Chemical Biology. (doi.org/10.1021/acschembio.2c00791)

Tal J. Shalev, Omnia Gamal El-Dien, Macaire M. S. Yuen, Lise van der Merwe, Matias Kirst, Alvin D. Yanchuk, Carol Ritland, John H. Russell, Joerg BohlmannGenetic architecture of terpene chemistry and growth traits and the impact of inbreeding on these traits in western redcedar (Thuja plicata). Evolutionary Applications (doi.org/10.1111/eva.13526)

2022

Tal J. Shalev, Omnia Gamal El-Dien, Macaire M.S. Yuen, Shu Shengqiang, Shaun D. Jackman, René L. Warren, Lauren Coombe, Lise van der Merwe, Ada Stewart, Lori B. Boston, Christopher Plott, Jerry Jenkins, Guifen He, Juying Yan, Mi Yan, Jie Guo, Jesse W. Breinholt, Leandro G. Neves, Jane Grimwood, Loren H. Rieseberg, Jeremy Schmutz, Inanc Birol, Matias Kirst, Alvin D. Yanchuk, Carol Ritland, John H. Russell, and Joerg Bohlmann. The western redcedar genome reveals low genetic diversity in a self-compatible conifer. Genome Research (doi: 10.1101/gr.276358.121)

Omnia Gamal El-DienTal J ShalevMacaire M S YuenRod StirlingLori D DanielsJesse W BreinholtLeandro G NevesMatias KirstLise Van der MerweAlvin D YanchukCarol RitlandJohn H RussellJoerg Bohlmann.  Genomic selection reveals hidden relatedness and increased breeding efficiency in western redcedar polycross breeding. Evolutionary Applications (doi: 10.1111/eva.13463)

Gagalova KK, Warren RI, Lauren Coombe L, Wong J, Nip KM, Yuen MMS, Whitehill JGA, Celedon JM, Ritland C, Taylor GA, Cheng D, Plettner P, Hammond SA, Mohamadi H, Zhao Y, Moore RA, Mungall AJ, Boyle B , Laroche J, Cottrell J, Mackay JJ, Lamothe M, Gérardi S, Isabel N, Pavy N, Jones SJM, Bohlmann J, Bousquet J, Birol I. Spruce giga-genomes: structurally similar yet distinctive with differentially expanding gene families and rapidly evolving genes. The Plant Journal (doi.org/10.1111/tpj.15889).

Chiu C and Bohlmann J.  Mountain Pine Beetle Epidemic: An Interplay of Terpenoids in Host Defense and Insect Pheromones. Annual Reviews of Plant Biology, (doi.org/10.1146/annurev-arplant-070921-103617)

Gagalova KK, Whitehill JGA, Culibrk L, Lin D, Lévesque-Tremblay V, Keeling CI, Coombe L, Birol I, Bohlmann J, and Jones SJM (2022) The genome of the forest insect pest Pissodes strobi reveals genome expansion and evidence of a Wolbachia endosymbiont​. G3, (doi.org/10.1093/g3journal/jkac038).

Murphy K, Poretsky E, Liu H, Micic N, Nyhuis A, Bohlmann J, Schmelz E, Zerbe P, Huffaker A, and N Bjarnholt (2022) Shielding the oil reserves: the scutellum as a source of chemical defenses. Plant Physiology (doi.org/10.1093/plphys/kiac038).

2021

Sunstrum FG, Liu HL, Jancsik S, Madilao LL, Bohlmann J, and Irmisch S.  4-Coumaroyl-CoA ligases in the biosynthesis of the anti-diabetic metabolite montbretin A. PLoS ONE doi.org/10.1371/journal.pone.0257478

Whitehill JGA, Yuen MMS, and J Bohlmann (2021) Constitutive and Insect-Induced Transcriptomes of Weevil-Resistant and Susceptible Sitka Spruce. Plant Environment Interactions, published online (doi.org/10.1002/pei3.10053).

Rosana ARR, Pokorny S, Klutsch JG, barra-Romero C, Sanichar R, Engelhardt D, van Belkum MJ, Erbilgin N, Bohlmann J, Carroll AL, and Vederas JC. Selection of entomopathogenic fungus Beauveria bassiana (Deuteromycotina: Hyphomycetes) for the biocontrol of Dendroctonus ponderosae(Coleoptera: Curculionidae, Scolytinae) in Western Canada. Applied Microbiology and Biotechnology. doi: 10.1007/s00253-021-11172-7

2020

Lo T, Coombe L, Lin D, Warren RL, Kirk H, Pandoh P, Zhao Y, Moore RA, Mungall AJ, Ritland C, Bousquet J, Jones SJM, Bohlmann J, Thomson A, and Birol I. Complete Chloroplast Genome Sequence of a Black Spruce (Picea mariana) from Eastern Canada. Microbiology Resource Announcements. doi: 10.1128/MRA.00877-20

Gülck T, Booth JK, Carvalho Â, Khakimov B, Crocoll C, Motawia MS, Møller BL, Bohlmann J, and Gallage NJ.  Synthetic Biology of Cannabinoids and Cannabinoid Glucosides in Nicotiana benthamiana and Saccharomyces cerevisiae. Journal of Natural Products. doi: 10.1021/acs.jnatprod.0c00241

Ding Y, Weckwerth PR, Poretsky E, Murphy KM, Sims J, Saldivar E, Christensen SA, Char SN, Yang B, Tong A, Shen Z, Kremling KA, Buckler ES, Kono T, Nelson DR, Bohlmann J, Bakker MG, Vaughan MM, Khalil AS, Betsiashvili M, Dressano K, Köllner TG, Briggs SP, Zerbe P, Schmelz EA, and Huffaker A. Genetic elucidation of interconnected antibiotic pathways mediating maize innate immunity. Nature Plants. doi: 10.1038/s41477-020-00787-9

Irmisch I, Jancsik S, Man Saint Yuen M, Madilao LL, and Bohlmann J. Complete Biosynthesis of the Anti-Diabetic Plant Metabolite Montbretin A. Plant Physiology. doi: 10.1104/pp.20.00522.

Beaulieu J, Nadeau S, Ding C, Celedon JM, Azaiez A, Ritland C, Laverdière JP, Deslauriers M, Adams G, Fullarton M, Bohlmann J, Lenz P, and Bousquet J. Genomic selection for resistance to spruce budworm in white spruce and relationships with growth and wood quality traits. Evolutionary Applications. doi: 10.1111/eva.13076.

Celedon JM, Whitehill JGA, Madilao LL, and Bohlmann J. Gymnosperm glandular trichomes: expanded dimensions of the conifer terpenoid defense system. Scientific reports. doi: 10.1038/s41598-020-69373-5.

Louca S, Rubin IN, Madilao LL, Bohlmann J, Doebeli M, and Wegener Parfrey L. Effects of forced taxonomic transitions on metabolic composition and function in microbial microcosms. Environmental Microbiology Reports. doi: 10.1111/1758-2229.12866.

Pelot KA, Mitchell R, Kwon M, Hagelthorn LM, Wardman JF, Chiang A, Bohlmann J, Ro DK, and Zerbe, P. Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase. Plant Journal. doi: 10.1111/tpj.13427.

Hamberger B, Man Saint Yuen M, Buschiazzo E, Cullis C, Yuen A, Ritland C, Bohlmann J, and Hamberger B. An Intact, But Dormant LTR Retrotransposon Defines a Moderately Sized Family in White Spruce (Picea glauca). The Spruce Genome. doi: 10.1007/978-3-030-21001-4_4.

Jackman SD, Coombe L, Warren RL, Kirk H, Trinh E, MacLeod T, Pleasance S, Pandoh P, Zhao Y, Coope RJ, Bousquet J, Bohlmann J, Jones SJM, and Birol I. Complete Mitochondrial Genome of a Gymnosperm, Sitka Spruce (Picea sitchensis), Indicates a Complex Physical Structure. Genome Biology Evolution. doi: 10.1093/gbe/evaa108.

Booth JK, Yuen MMS, Jancsik S, Madilao L, Page J, and Bohlmann J. Terpene Synthases and Terpene Variation in Cannabis sativa. Plant Physiology. doi: 10.1104/pp.20.00593.

Parent G, Méndez‐Espinoza C, Giguère I, Mageroy MH, Charest M, Bauce E, Bohlmann J, and MacKay J. Hydroxyacetophenone defenses in white spruce against spruce budworm. Evolutionary Applications. doi: 10.1111/eva.12885.

Livingston SJ, Quilichini TD, Booth JK, Wong DCJ, Rensing KH, Laflamme-Yonkman J, Castellarin SD, Bohlmann J, Page JE, and Samuels AL. Cannabis glandular trichomes alter morphology and metabolite content during flower maturation. Plant Journal. doi: 10.1111/tpj.14516.

2019

Irmisch S, Jancsik S, Yuen MMS, Madilao LL, and Bohlmann J. Biosynthesis of the anti-diabetic metabolite montbretin A: glucosylation of the central intermediate mini-MbA. Plant Journal. doi: 10.1111/tpj.14493.

Ding Y, Murphy K, Poretsky E, Mafu S, Yang B, Char SN, Christensen S, Saldivar E, Wu M, Wang Q, Ji L, Schmitz R, Kremling K, Buckler E, Shen Z, Briggs S, Bohlmann J, Sher A, Castro-Falcón G, Hughes C, Huffaker A, Zerbe P, and Schmelz E. Multiple genes recruited from hormone pathways partition maize diterpenoid defenses. Nature Plants. doi: 10.1038/s41477-019-0509-6.

Irmisch S, Ruebsam H, Jancsik S, Yuen MMS, Madilao LL, and Bohlmann J. Flavonol biosynthesis genes and their use in engineering the plant antidiabetic metabolite montbretin A. Plant Physiology. doi: 10.1104/pp.19.00254.

Lin D, Coombe L, Jackman S, Gagalova K, Warren R, Hammond S, McDonald H, Kirk H, Pandoh P, Zhao Y, Moore R, Mungall A, Ritland C, Doerksen T, Jaquish B, Bousquet J, Jones S, Bohlmann J, and Birol I. Complete chloroplast genome sequence of an Engelmann spruce (Picea engelmannii) genotype from western Canada. Microbiology Resource Announcements. doi: 10.1128/MRA.00382-19.

Lin D, Coombe L, Jackman S, Gagalova K, Warren R, Hammond S, Kirk H, Pandoh P, Zhao Y, Moore R, Mungall A, Ritland C, Doerksen T, Jaquish B, Isabel N, Bousquet J, Jones S, Bohlmann J, and Birol I. Complete chloroplast genome sequence of a white spruce (Picea glauca, genotype WS77111) from eastern Canada. Microbiology Resource Announcements. doi: 10.1128/MRA.00381-19.

Celedon J and Bohlmann J. Oleoresin defenses in conifers: chemical diversity, terpene synthases, and limitations of oleoresin defense under climate change. New Phytologisty. doi: 10.1111/nph.15984.

Chiu CC, Keeling CI, Henderson HM, and Bohlmann J. Functions of mountain pine beetle cytochromes P450 CYP6DJ1, CYP6BW1 and CYP6BW3 in the oxidation of pine monoterpenes and diterpene resin acids. PLoS ONE. doi: 10.1371/journal.pone.0216753.

Whitehill JGA and Bohlmann J. A molecular and genomic reference system for conifer defense against insects. Plant, Cell & Environ. doi: 10.1111/pce.13571.

Chiu CC, Keeling CI, and Bohlmann J. Cytochromes P450 preferentially expressed in antennae of the mountain pine beetle. J Chem Ecol. doi: 10.1007/s10886-018-0999-0.

Jackman SD, Coombe L, Warren RL, Kirk H, Trinh E, McLeod T, Pleasance S, Pandoh P, Zhao Y, Coope RJ, Bousquet J, and Bohlmann J. Largest complete mitochondrial genome of a gymnosperm, Sitka spruce (Picea sitchensis), indicates complex physical structure. bioRxiv. doi: 10.1101/601104.

Warren RL, Coombe L, Mohamadi H, Zhang J, Jaquish B, Isabel N, Jones SJM, Bousquet J, Bohlmann J, and Birol I. ntEdit: scalable genome sequence polishing. Bioinformatics btz400. doi: 10.1093/bioinformatics/btz400.

Booth JK and Bohlmann J. Terpenes in Cannabis sativa – From plant genome to humans. Plant Science. doi: 10.1016/j.plantsci.2019.03.022.

Chiu CC, Keeling CI, and Bohlmann J. The cytochrome P450 CYP6DE1 catalyzes the conversion of α-pinene into the mountain pine beetle aggregation pheromone trans-verbenol. Sci Rep-UK. doi: 10.1038/s41598-018-38047-8.

Whitehill JGA, Yuen MMS, Henderson H, Madilao L, Kshatriya K, Bryan J, Jaquish B, and Bohlmann J. Functions of stone cells and oleoresin terpenes in the conifer defense syndrome. New Phytologist. doi: 10.1111/nph.15477.

2018

Henry LK, Thomas ST, Widhalm JR, Lynch JH, Davis TC, Kessler SA, Bohlmann J, Noel JP, and N Dudareva. Contribution of isopentenyl phosphate to plant terpenoid metabolism. Nature Plants. doi: 10.1038/s41477-018-0220-z.

Kshatriya K, Whitehill JGA, Madilao L, Henderson H, Kermode A, Dolotelo D, and Bohlmann J. Histology of resin vesicles and oleoresin terpene composition of conifer seeds. Can J Forest Res. doi: 10.1139/cjfr-2018-0164.

Shalev TJ, Yuen MMS, Gesell A, Yuen A, Russell JH,and Bohlmann J. An annotated transcriptome of highly inbred Thuja plicata (Cupressaceae) and its utility for gene discovery of terpenoid biosynthesis and conifer defense. Tree Genet & Genomes. doi: 10.1007/s11295-018-1248-y.

Chiu CC, Keeling CI, and Bohlmann J. Monoterpenyl esters in juvenile mountain pine beetle and sex-specific release of the aggregation pheromone trans-verbenol. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1722380115.

Parent GJ, Giguère I, Mageroy M, Bohlmann J, and MacKay JJ. Evolution of the biosynthesis of two hydroxyacetophenones in plants. Plant, Cell & Environ. doi: 10.1111/pce.13134.

Celedon JM and Bohlmann J. An extended model of heartwood secondary metabolism informed by functional genomics. Tree physiol. doi: 10.1093/treephys/tpx070.

Roach MJ, Johnson D, Bohlmann J, van Vuuren H, Jones S, Pretorius I, Schmidt SA, and Borneman AR. Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay. PLOS Genet. doi: 10.1371/journal.pgen.1007807.

Irmisch S, Jo S, Roach CR, Jancsik S, Yuen MMS, Madilao L, O’Neil-Johnson MM, Williams R, Withers SG, and Bohlmann J. Discovery of UDP-glycosyltransferases and BAHD-acyltransferases in the biosynthesis of the anti-diabetic plant metabolite montbretin A. Plant Cell. doi: 10.1105/tpc.18.00406.

Jackman SD, Coombe L, Chu J, Warren RL, Vandervalk BP, Yeo S, Xue Z, Mohamadi H, Bohlmann J, Jones SJM, and Birol I. Tigmint: Correcting assembly errors using linked reads from large molecules. BMC Bioinformatics. doi: 10.1186/s12859-018-2425-6.

Mafu S, Ding Y, Murphy KM, Yaacoobi O, Addison JB, Wang Q, Shen Z, Briggs SP, Bohlmann J, Castro-Falcón G, Hughes CC, Betsiashvili M, Huffaker A, Schmelz EA, and Zerbe P. Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize. Plant Physiol. doi: 10.1104/pp.17.01351.

2017

Celedon JM, Yuen MMS, Chiang A, Henderson H, Reid KE, and Bohlmann J. Cell-type and tissue specific transcriptomes of the white spruce (Picea glauca) bark unmask fine-scale spatial patterns of constitutive and induced conifer defense. Plant J. doi: 10.1111/tpj.13673.

Mageroy MH, Jancsik S, Yuen MMS, Fischer M, Withers SG, P aetz C, Schneider B, and Bohlmann J. A conifer UDP-sugar dependent glycosyltransferase contributes to acetophenone metabolism and defense against insects. Plant Physiol. doi: 10.1104/pp.17.00611.

Chiu CC, Keeling CI, and Bohlmann J. Toxicity of pine monoterpenes to mountain pine beetle. Sci Rep-UK. doi: 10.1038/s41598-017-08983-y.

Mageroy MH, Lachance D, Jancsik S, Parent G, Séguin A, MacKay J, and Bohlmann J. In vivo function of Pgßglu-1 in the release of acetophenones in white spruce. PeerJ. doi: 10.7717/peerj.3535.

Moniodis J, Jones CG, Renton M, Plummer JA, Barbour EL, Ghisalberti EL, and Bohlmann J. Sesquiterpene variation in West Australian Sandalwood (Santalum spicatum). Molecules. doi: 10.3390/molecules22060940.

Burke J, Bohlmann J, and Carroll A. Consequences of distributional asymmetry in a warming environment: invasion of novel forests by the mountain pine beetle. Ecosphere. doi: 10.1002/ecs2.1778.

Booth JK, Page JE and Bohlmann J. Terpene synthases from Cannabis sativa. PLoS ONE. doi: 10.1371/journal.pone.0173911.

Pavy N, Lamothe M, Pelgas B, Gagnon F, Birol I, Bohlmann J, Mackay J, Isabel N and Bousquet, J. A high resolution reference genetic map positioning 8.8K genes for the conifer white spruce: Structural genomics implications and correspondence with physical distance. Plant J. doi: 10.1111/tpj.13478.

Mafu S, Karunanithi PS, Palazzo TA, Harrod BL, Rodriguez SM, Mollhoff IN, O’Brien TE, Tong S, Fiehn O, Tantillo DJ, Bohlmann J and Zerbe P. Biosynthesis of the microtubule-destabilizing diterpene pseudolaric acid B from golden larch involves an unusual diterpene synthase. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1612901114.

Pelot KA, Mitchell R, Kwon M, Hagelthorn DM, Wardman JF, Chiang A, Bohlmann J, Ro DK and Zerbe P. Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase. Plant J. doi: 10.1111/tpj.13427.

2016

Coombe L, Warren RL, Yang C, Vandervalk BP, Mayo M, Moore RA, Pleasance S, Coope RJ, Bohlmann J, Holt RA, Jones SJM, and I Birol. Assembly of the complete Sitka spruce chloroplast genome using 10X Genomics’ GemCode sequencing data. PLoS ONE. doi: 10.1371/journal.pone.0163059.

Whitehill JGA, Henderson H, Strong W, Jaquish B, and Bohlmann J. Function of Sitka spruce stone cells as a physical defense against white pine weevil. Plant, Cell & Environ. doi: 10.1111/pce.12810.

Robert JA, Bonnett T, Pitt C, Spooner LJ, Fraser J, Yuen MMS, Keeling CI, Bohlmann J, and Huber DPW. Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development. PeerJ. doi: 10.7717/peerj.2109.

Celedon JM and Bohlmann J. Genomics-based discovery of plant genes for synthetic biology of terpenoid fragrances: A case study in sandalwood oil biosynthesis. Methods Enzymol. doi: 10.1016/bs.mie.2016.03.008.

Celedon JM, Chiang A, Yuen MM, Diaz-Chavez ML, Madilao LL, Finnegan PM, Barbour EL, and Bohlmann J. Heartwood specific transcriptome and metabolite signatures of tropical sandalwood (Santalum album) reveal the final step of (Z)-santalol fragrance biosynthesis. Plant J. doi: 10.1111/tpj.13162.

Geisler, K, Jensen NB, Yuen MMS, Madilao L, and Bohlmann J. Modularity of conifer diterpene resin acid biosynthesis: P450 enzymes of different CYP720B clades use alternative substrates and converge on the same products. Plant Physiol. doi: 10.1104/pp.16.00180.

Jackman SD, Warren RL, Gibb EA, Vandervalk BP, Mohamadi H, Chu J, Raymond A, Pleasance S, Coope R, Wildung MR, Ritland CE, Bousquet  J, Jones, SJM, Bohlmann J, and Birol I. Organellar genomes of white spruce (Picea glauca): Assembly and annotation. Genome Biol Evol. doi: 10.1093/gbe/evv244.

Andersen-Ranberg J, Kongstad KT, Nielsen MT, Jensen NB, Pateraki I, Bach SS, Hamberger B, Zerbe P, Staerk D, Bohlmann J, Møller BL, and Hamberger B. Expanding the landscape of diterpene structural diversity through stereochemically controlled combinatorial biosynthesis. Angewandte Chemie. doi: 10.1002/ange.201510650.

Keeling CI, Li M, Sandhu HK, Henderson H, Yuen MMS, and Bohlmann J. Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis. Insect Biochem Molec. doi: 10.1016/j.ibmb.2016.01.002.

2015

Whitehill JGA, Henderson H, Schuetz M, Skyba O, Yuen MMS, King J, Samuels AL, Mansfield SD, and Bohlmann J. Histology and cell wall biochemistry of stone cells in the physical defense of conifers against insects. Plant, Cell & Environ. doi: 10.1111/pce.12654.

Zerbe P, Rodriguez SM, Mafu S, Chiang A, Sandhu HK, O’Neil-Johnson M, Starks CM, and Bohlmann J. Exploring diterpene metabolism in non-model species: Transcriptome-enabled discovery and functional characterization of labda-7, 13E-dienyl diphosphate synthase from Grindelia robusta. Plant J. doi: 10.1111/tpj.12925.

Warren RL, Keeling CI, Yuen MMS, Raymond A, Taylor GA, Vandervalk BP, Mohamadi H, Paulino D, Chiu Readman, Jackman SD, Robertson G, Yang C, Hoffmann M, Weigel D, Nelson DR, Ritland C, Isabel N, Jaquish B, Yanchuk A, Bousquet J, Jones SJM, MacKay J, Birol I, and Bohlmann J. Improved white spruce (Picea glauca) genome assemblies and annotation of large gene families of conifer terpenoid and phenolic defense metabolism. Plant J. doi: 10.1111/tpj.12886.

Zerbe P and Bohlmann J. Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering. Trends Biotechnol. doi: 10.1016/j.tibtech.2015.04.006.

Schrader J and Bohlmann J. Editors. Advances in biochemical engineering/biotechnology: biotechnology of isoprenoids. Springer Berlin Heidelberg, online, doi: 10.1007/978-3-319-20107-8.

Zerbe P and Bohlmann J. Enzymes for synthetic biology of ambroxide-related diterpenoid fragrance compounds. In Advances in biochemical engineering/biotechnology: biotechnology of isoprenoids 427–447. Springer Berlin Heidelberg, online, doi: 10.1007/10_2015_308.

Bohlmann J, Gesell A, Blaukopf M, Madilao L, Yuen MMS, Withers SG, Mattsson J, and Russell JH. The gymnosperm cytochrome P450 CYP750B1 catalyzes stereospecific monoterpene hydroxylation of (+)-sabinene in thujone biosynthesis in Thuja plicata. Plant Physiol. doi: 10.1104/pp.15.00315.

Moniodis J, Jones CG, Barbour EL, Plummer JA, Ghisalberti EL, and Bohlmann J. The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum). Phytochemistry. doi: 10.1016/j.phytochem.2014.12.009.

Mageroy MH, Parent G, Germanos G, Giguère I, Delvas N, Maaroufi H, Bauce É, Bohlmann J, and MacKay J. Expression of the β‐glucosidase gene Pgβglu‐1 underpins natural resistance of white spruce against spruce budworm. Plant J. doi: 10.1111/tpj.12699.

Novak M, Lah L, Šala M, Stojan J, Bohlmann J, and Komel R. Oleic acid metabolism via a conserved cytochrome P450 system-mediated ω-hydroxylation in the bark beetle-associated fungus Grosmannia clavigera. PloS One. doi: 10.1371/journal.pone.0120119.

Külheim C, Jones CG, Plummer JA, Ghisalberti EL, Barbour L, and Bohlmann J. Foliar application of methyl jasmonate does not increase terpenoid accumulation, but weakly elicits terpenoid pathway genes in sandalwood (Santalum album L.) seedlings. Plant Biotechnol. doi: 10.5511/plantbiotechnology.14.1014a.

2014

Appel HM, Fescemyer H, Ehlting J, Weston D, Rehrig E, Joshi T, Xu D, Bohlmann J, and Schultz J. Transcriptional responses of Arabidobsis thaliana to chewing and sucking insect herbivores. Frontiers Plant Sci. doi: 10.3389/fpls.2014.00565.

Pitt C, Robert JA, Bonnett TR, Keeling CI, Bohlmann J, and Huber DPW. Proteomics indicators of the rapidly shifting physiology from whole mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), adults during early host colonization. PloS One. doi: 10.1371/journal.pone.0110673.

De La Torre A, Birol I, Bousquet J, Ingvarsson P, Jansson S, Jomes SJM, Keeling CI, MacKay J, Nilsson O, Ritland K, Street N, Yanchuk A, Zerbe P, and Bohlmann J. Insights into conifer giga-genomes. Plant Physiol. doi: 10.1104/pp.114.248708.

Jackman SD, Bohlmann J, and Birol I. UniqTag: Content-derived unique and stable identifiers for gene annotation. BioRxIV. doi: 10.1101/007583.

Roach C, Hall DE, Zerbe P, and Bohlmann J. Plasticity and evolution of (+)-3-Carene Synthase and (-)-Sabinene synthase functions of a Sitka spruce monoterpene synthase gene family associated with weevil resistance. J Biol Chem. doi: 10.1074/jbc.M114.571703.

Zerbe P, Chiang A, Dullat H, O-Neil-Johnson M, Starks C, Hamberger B, and Bohlmann J. Diterpene synthases of the biosynthetic system of medicinally active diterpenoids in Marrubium vulgare. Plant J. doi: 10.1111/tpj.12589.

Wang Y, Lim L, Madilao L, Lah L, Bohlmann J, and Breuil C. Gene discovery for enzymes involved in limonene modification or utilization by the mountain pine beetle-associated pathogen Grosmannia clavigera. Appl Environ Microb. doi: 10.1128/AEM.00670-14.

Zerbe P and Bohlmann J. Bioproducts, Biofuels, and Perfumes: Conifer Terpene Synthases and their Potential for Metabolic Engineering. In Phytochemicals—Biosynthesis, Function and Application. R. Jetter (Ed.). Springer Publications. doi: 10.1007/978-3-319-04045-5_5.

Janes JK, Li Y, Keeling CI, Yuen MMS, Boone CK, Cooke JEK, Bohlmann J, Huber DPW, Murray BW, Coltman DW, and Sperling FAH. How the mountain pine beetle (Dendroctonus ponderosae) breached the Canadian Rocky Mountains. Mol Biol Evol. doi: 10.1093/molbev/msu135.

Sena JS, Giguère I, Boyle B, Rigault P, Birol I, Zuccolo A, Ritland K, Ritland C, Bohlmann J, Jones S, Bousquet J, and Mackay J. Evolution of gene structure in the conifer Picea glauca: a comparative analysis of the impact of intron size. BMC Plant Biology. doi: 10.1186/1471-2229-14-95.

Alamouti SM, Haridas S, Feau N, Robertson G, Bohlmann J, and Breuil C. Comparative genomics of the pine pathogens and beetle symbionts in the genus Grosmannia. Mol Biol Evol. doi: 10.1093/molbev/msu102.

Kolosova N, Breuil C, and Bohlmann J. Cloning and characterization of chitinases from interior spruce and lodgepole pine. Phytochemistry. doi: 10.1016/j.phytochem.2014.02.006.

Hammerbacher A, Paetz C, Wright LP, Fischer TC, Bohlmann J, Davis AJ, Fenning TM, Gershenzon J, and Schmidt A. Flavan-3-ols in Norway spruce: Biosynthesis, accumulation and function in response to attack by the bark beetle-associated fungus Ceratocystis polonica. Plant Physiol. doi: 10.1104/pp.113.232389.

Pateraki I, Andersen-Ranberg J, Hamberger B, Heskes AM, Martens HJ, Zerbe P, Spanner Bach S, Lindberg Møller B, Bohlmann J, and Hamberger B. Manoyl oxide (13R), the biosynthetic precursor of forskolin, is synthesized in specialized root cork cells in Coleus forskohlii. Plant Physiol. doi: 10.1104/pp.113.228429.

Novak M, Krajnc AU, Lah L, Zupanec N, Krasevec N, Krizman M, Bohlmann J, and Komel R. Low-density Ceratocystis polonica inoculation of Norway spruce (Picea abies) triggers accumulation of monoterpenes with antifungal properties. Eur J Forest Res. doi: 10.1007/s10342-013-0772-4.

2013

Robert JA, Pitt C, Bonnett TR, Yuen MMS, Keeling CI, Bohlmann J, and Huber DPW. Disentangling detoxification: gene expression analysis of feeding mountain pine beetle illuminates molecular-level host chemical defense detoxification mechanisms. PloS One. doi: 10.1371/journal.pone.0077777.

Kadow D, Bohlmann J, Philips Mora W, Rohn S, and Lieberei R. Identification of two genotypes of cacao tree (Theobroma cacao L.). J Appl Bot Food Qual. doi: 10.5073/JABFQ.2013.086.013.

Keeling CI, Chiu CC, Aw T, Li M, Henderson H, Tittiger C, Weng H-B, Blomquist GJ, and Bohlmann J. Frontalin pheromone biosynthesis in the mountain pine beetle, Dendroctonus ponderosae, and the role of isoprenyl diphosphate synthases. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1316498110.

Keeling CI, Henderson H, Li M, Dullat HK, Ohnishi T, and Bohlmann J. CYP345E2, an antenna-specific cytochrome P450 from the mountain pine beetle, Dendroctonus ponderosae Hopkins, catalyses the oxidation of pine host monoterpene volatiles. Insect Biochem Molec. doi: 10.1016/j.ibmb.2013.10.001.

Diaz-Chavez ML, Moniodis J, Madilao LL, Jancsik S, Keeling CI, Barbour EL, Ghisalberti EL, Plummer JA, Jones CG, and Bohlmann J. Biosynthesis of sandalwood oil: Santalum album CYP76F cytochromes P450 produce santalols and bergamotol. PLoS One. doi: 10.1371/journal.pone.0075053.

Kadow D, Bohlmann J, Philips W, and Lieberei R. Identification of main fine flavour components in two genotypes of the chocolate tree (Theobroma cacao L.). J Appl Bot Food Qual. doi: 10.5073/JABFQ.2013.086.013.

Boone CK, Keefover-Ring K, Mapes AC, Adams AS, Bohlmann J, and Raffa KF. Bacteria associated with a tree-killing insect reduce concentrations of plant defense compounds. J Chem Ecol. doi: 10.1007/s10886-013-0313-0.

Birol I, Raymond A, Jackman SD, Pleasance S, Coope R, Taylor GA, Yuen MM, Keeling CI, Brand D, Vandervalk BP, Kirk H, Pandoh P, Moore RA, Zhao Y, Mungall AJ, Jaquish B, Yanchuk A, Ritland C, Boyle B, Bousquet J, Ritland K, Mackay J, Bohlmann J, and Jones SJ. Assembling the 20 Gb white spruce (Picea glauca) genome from whole-genome shotgun sequencing data. Bioinformatics. doi: 10.1093/bioinformatics/btt178.

Haridas S, Wang Y, Lim L, Alamouti SM, Jackman S, Docking R, Robertson G, Birol I, Bohlmann J, and Breuil C. The genome and transcriptome of the pine saprophyte Ophiostoma Piceae, and a comparison with the bark beetle-associated pine pathogen Grosmannia clavigera. BMC Genomics. doi: 10.1186/1471-2164-14-373.

Hammerbacher A, Schmidt A, Wadke N, Wright LP, Schneider B, Bohlmann J, Brand WA, Fenning TM, Gershenzon J, and Paetz C. A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce. Plant Physiol. doi: 10.1104/pp.113.218610.

Zerbe P, Hamberger B, Yuen MM, Chiang A, Sandhu HK, Madilao LL, Nguyen A, Hamberger B, Bach SS, and Bohlmann J. Gene discovery of modular diterpene metabolism in nonmodel systems. Plant Physiol. doi: 10.1104/pp.113.218347.

Nystedt B, Street NR, Wetterbom A, Zuccolo A, Lin YC, Scofield DG, Vezzi F, Delhomme N, Giacomello S, Alexeyenko A, Vicedomini R, Sahlin K, Sherwood E, Elfstrand M, Gramzow L, Holmberg K, Hällman J, Keech O, Klasson L, Koriabine M, Kucukoglu M, Käller M, Luthman J, Lysholm F, Niittylä T, Olson A, Rilakovic N, Ritland C, Rosselló JA, Sena J, Svensson T, Talavera-López C, Theißen G, Tuominen H, Vanneste K, Wu ZQ, Zhang B, Zerbe P, Arvestad L, Bhalerao R, Bohlmann J, Bousquet J, Garcia Gil R, Hvidsten TR, de Jong P, MacKay J, Morgante M, Ritland K, Sundberg B, Thompson SL, Van de Peer Y, Andersson B, Nilsson O, Ingvarsson PK, Lundeberg J, and Jansson S. The Norway spruce genome sequence and conifer genome evolution. Nature. doi: 10.1038/nature12211.

Hall DE, Yuen MMS, Jancsik S, Lara Quesada A, Dullat HK, Li M, Henderson H, Arango-Velez A, Liao NY, Docking TR, Chan SK, Cooke JEK, Breuil C, Keeling CI, and Bohlmann J. Transcriptome resources and functional characterization of monoterpene synthases for two host species of the mountain pine beetle, lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana).  BMC Plant Biol. doi: 10.1186/1471-2229-13-80.

Xiao M, Zhang Y, Chen X, Lee EJ, Barber CJ, Chakrabarty R, Desgagné-Penix I, Haslam TM, Kim YB, Liu E, Macnevin G, Masada-Atsumi S, Reed DW, Stout JM, Zerbe P, Zhang Y, Bohlmann J, Covello PS, De Luca V, Page JE, Ro DK, Martin VJ, Facchini PJ, and Sensen CW. Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest. J Biotechnol. doi: 10.1016/j.jbiotec.2013.04.004.

Foster AJ, Hall DE, Mortimer L, Abercromby S, Gries R, Gries G, Bohlmann J, Russell J, and Mattsson J. Identification of Genes in Thuja plicata Foliar Terpenoid Defenses. Plant Physiol. doi: 10.1104/pp.112.206383.

Andersson MN, Grosse-Wilde E, Keeling CI, Bengtsson JM, Yuen MM, Li M, Hillbur Y, Bohlmann J, Hansson BS, and Schlyter F. Antennal transcriptome analysis of the chemosensory gene families in the tree killing bark beetles, Ips typographus and Dendroctonus ponderosae(Coleoptera: Curculionidae: Scolytinae). BMC Genomics. doi: 10.1186/1471-2164-14-198.

Janocha S, Zapp J, Hutter M, Kleser M, Bohlmann J, and Bernhardt R. Resin acid conversion with CYP105A1: an enzyme with potential for the production of pharmaceutically relevant diterpenoids. ChemBioChem. doi: 10.1002/cbic.201200729.

Keeling CI, Yuen MMS, Liao NY, Docking TR, Chan SK, Taylor GA, Palmquist DL, Jackman SD, Nguyen A, Li M, Henderson H, Janes JK, Zhao Y, Pandoh P, Moore R, Sperling FAH, Huber DPW, Birol I, Jones SJM, and Bohlmann J. Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest. Genome Biol. doi: 10.1186/gb-2013-14-3-r27.

Tsui CK, DiGuistini S, Feau N, Wang Y, Bohlmann J, and Hamelin RC. Unequal recombination and evolution of the mating-type (MAT) loci in the pathogenic fungus Grosmannia clavigera and relatives. G3: Genes, Genomes, Genetics. doi: 10.1534/g3.112.004986.

Boone CK, Keefover-Ring K, Mapes AC, Adams AS, Bohlmann J, and Raffa KF. Bacteria Associated with a Tree-Killing Insect Reduce Concentrations of Plant Defense Compounds. J Chem Ecol. doi: 10.1007/s10886-013-0313-0.

Reid K, Holliday J, Yuen M, Nguyen A, Aitken S, and Bohlmann J. Sequencing of Sitka spruce (Picea sitchensis) cDNA libraries constructed from autumn buds and foliage reveals autumn-specific spruce transcripts. Tree Genet & Genomes. doi: 10.1007/s11295-012-0584-6.

Hall DE, Zerbe P, Jancsik S, Lara Quesada A, Dullat H, Madilao LL, Yuen M, and Bohlmann J. Evolution of Conifer Diterpene Synthases: Diterpene Resin Acid Biosynthesis in Lodgepole Pine and Jack Pine Involves Monofunctional and Bifunctional Diterpene Synthases. Plant Physiol. doi: 10.1104/pp.112.208546.

Wang Y, Lim L, DiGuistini S, Robertson G, Bohlmann J, and Breuil C. A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees. New Phytol. doi: 10.1111/nph.12063.

Lah L, Haridas S, Bohlmann J, and Breuil C. The cytochromes P450 of Grosmannia clavigera: Genome organization, phylogeny, and expression in response to pine host chemicals. Fungal Genet Biol. doi: 10.1016/j.fgb.2012.10.002.

2012

Bonnett TR, Robert JA, Pitt C, Fraser JD, Keeling CI, Bohlmann J, and Huber DPW. Global and comparative proteomic profiling of overwintering and developing mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae) larvae. Insect Biochem Molec. doi: 10.1016/j.ibmb.2012.08.003.

Keeling CI, Henderson H, Li M, Yuen M, Clark EL, Fraser JD, Huber DPW, Liao NY, Docking TR, Birol I, Chan SK, Taylor GA, Palmquist D, Jones SJM, and Bohlmann J. Transcriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests. Insect Biochem Molec. doi: 10.1016/j.ibmb.2012.03.010.

Martin DM, Chiang A, Lund ST, and Bohlmann J. Biosynthesis of wine aroma: transcript profiles of hydroxymethylbutenyl diphosphate reductase, geranyl diphosphate synthase, and linalool/nerolidol synthase parallel monoterpenol glycoside accumulation in Gewürztraminer grapes. Planta. doi: 10.1007/s00425-012-1704-0.

Khadempour L, Lemay V, Jack D, Bohlmann J, and Breuil C. The relative abundance of mountain pine beetle fungal associates changes through the beetle life cycle in pine trees. Microb Ecol. doi: 10.1007/s00248-012-0077-z.

Bohlmann J. Pine terpenoid defences in the mountain pine beetle epidemic and in other conifer pest interactions: specialized enemies are eating holes into a diverse, dynamic and durable defence system. Tree Physiol. doi: 10.1093/treephys/tps065.

Caniard A, Zerbe P, Legrand S, Cohade A, Valot N, Magnard J-L, Bohlmann J, and Legendre L. Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture. BMC Plant Biol. doi: 10.1186/1471-2229-12-119.

Kelleher CT, Wilkin J, Zhuang J, Cortés AJ, Pérez Quintero AL, Gallagher TF, Bohlmann J, Douglas CJ, Ellis BE, and K. Ritland. SNP discovery, gene diversity, and linkage disequilibrium in wild populations of Populus tremuloides. Tree Genet & Genomes. doi: 10.1007/s11295-012-0467-x.

Tsui CKM, Amanda R, El-Kassaby Y, Rice A, Alamouti SM, Sperling F, Cooke J, Bohlmann J, and R Hamelin. Population structure and migration pattern of a conifer pathogen, Grosmannia clavigera, as influenced by its symbiont, the mountain pine beetle. Mol Ecol. doi: 10.1111/j.1365-294X.2011.05366.x.

Buschiazzo E, Ritland C, Bohlmann J, and K Ritland. Slow but not low: genomic comparisons reveals slower evolutionary rate and higher dN/dS in conifers compared to angiosperms. BMC Evol Biol. doi: 10.1186/1471-2148-12-8.

Facchini PJ, Bohlmann J, Covello PS, De Luca V, Mahadevan R, Page JE, Ro D-K, Sensen CW, Storms R, and VJJ Martin. Synthetic biosystems for the production of high-value plant metabolites. Trends Biotechnol (featured on the April 2012 cover of Trends in Biotechnology). doi: 10.1016/j.tibtech.2011.10.001.

Zerbe P, Chiang A, Yuen M, Hamberger B, Hamberger Br, Draper JA, Britton R, and Bohlmann J. Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production. J Biol Chem. doi: 10.1074/jbc.M111.317669.

Kolosova N and Bohlmann J. Conifer defense against insects and fungal pathogens. Submitted for publication in: Growth and Defence in Plants: Resource Allocation at Multiple Scales, Editor R. Matyssek, Springer. doi: 10.1007/978-3-642-30645-7_4.

Zerbe P, Chiang A, and Bohlmann J. Mutational analysis of white spruce (Picea glauca) ent-kaurene synthase (PgKS) reveals common and distinct mechanisms of conifer diterpene synthases of general and specialized metabolism. Phytochemistry. doi: 10.1016/j.phytochem.2011.11.004.

2011

Bohlmann J. Terpenoid synthases-from chemical ecology and forest fires to biofuels and bioproducts. Structure. doi: 10.1016/j.str.2011.11.009.

Keeling CI, Weißhaar S, Ralph SG, Jancsik S, Hamberger Br, Dullat HK, and Bohlmann J. Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp.). BMC Plant Biol. doi: 10.1186/1471-2229-11-43.

Diguistini S, Wang Y, Liao N, Taylor G, Tanguay P, Feau N, Henrissat B, Chan S, Hesse-Orce U, Massoumi Alamouti S, Tsui CKM, Docking R, Levasseur A, Robertson G, Birol I, Holt R, Marra M, Hirst M, Hamelin R, Jones S, Bohlmann J, and C Breuil. Genome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1011289108.

Hall DE, Robert JA, Keeling CI, Domanski D, Quesada AL, Jancsik S, Kuzyk M, Hamberger Br, Borchers CH, and Bohlmann J. An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil. Plant J. doi: 10.1111/j.1365-313X.2010.04478.x.

Chen F, Tholl D, Bohlmann J, and E Pichersky. The family of terpene synthases in plants: A mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom. Plant J. doi: 10.1111/j.1365-313X.2011.04520.x.

Antunes LCM, Arena ET, Menendez A, Han J, Ferreira RBR, Buckner MCC, Loli P, Madilao LL, Bohlmann J, Borchers CH and BB Finlay. The impact of Salmonella infection on host hormone metabolism revealed by metabolomics. Infect Immun. doi: 10.1128/IAI.01373-10.

Stephenson PG, Harris N, Cottrell JE, Ralph SG, Bohlmann J, and G Taylor. A transcriptomic approach to identify genes associated with wood density in Picea sitchensis. Scand J Forest Res. doi: 10.1080/02827581.2011.564405.

Samarasekera N, Keeling CI, Bohlmann J, and B Murray. Isolation and characterization of EST-derived microsatellite markers for the mountain pine beetle (Dendroctonus ponderosae Hopkins). Mol Ecol Resour. doi: 10.1111/j.1755-0998.2011.03004.x.

Jones CG, Moniodis J, Zulak KG, Scaffidi A, Plummer JA, Ghisalberti EL, Barbour EL, and Bohlmann J. Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both the TPS-a and TPS-b subfamilies, including santalene synthases. J Biol Chem. doi: 10.1074/jbc.M111.231787.

Keeling CI, Madilao LL, Zerbe P, Dullat HK, and Bohlmann J. The primary diterpene synthase products of Picea abies levopimaradiene/abietadiene synthase (PaLAS) are epimers of a thermally unstable diterpenol. J Biol Chem. doi: 10.1074/jbc.M111.245951.

Boone CK, Aukema BH, Bohlmann J, Carroll AL, and Raffa KF. Efficacy of tree defense physiology varies with bark beetle population density: a basis for positive feedback in eruptive species. Can J Forest Res. doi: 10.1139/x11-041.

Alamouti SM, Wang V, DiGuistini S, Six DL, Bohlmann J, Hamelin RC, Feau N, and C Breuil. Gene genealogies reveal cryptic species and host preference for the pine fungal pathogen Grosmannia clavigera. Mol Ecol. doi: 10.1111/j.1365-294X.2011.05109.x.

Haridas S, Breuill C, Bohlmann J, and T Hsiang. A biologist’s guide to de novo genome assembly using next-generation sequence data: A test with fungal genomes. J Microbiol Meth. doi: 10.1016/j.mimet.2011.06.019.

Adams A, Boone CK, Bohlmann J and K Raffa. Responses of bark beetle-associated bacteria to host monoterpenes and their relationship to insect life histories. J Chem Ecol. doi: 10.1007/s10886-011-9992-6.

Lah L, Podobnik B, Novak M, Korošec B, Berne S, Vogelsang M, Kraševec N, Zupanec N, Stojan J, Bohlmann J, and R Komel. The versatility of the fungal cytochrome P450 monooxygenase system is instrumental in xenobiotic detoxification. Mol Microbiol. doi: 10.1111/j.1365-2958.2011.07772.x.

Hammerbacher A, Ralph SG, Bohlmann J, Fenning TM, Gershenzon J, and A Schmidt. Biosynthesis of the major tetrahydroxystilbenes in spruce, astringin and isorhapontin, proceeds via resveratrol and is enhanced by fungal infection. Plant Physiology. doi: 10.1104/pp.111.181420.

Bleeker P, Spyropoulou E, Diergaarde P, Volpin H, De Both MTJ, Zerbe P, Bohlmann J, Falara V, Matsuba Y, Pichersky E, Haring M, and R Schuurink. RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes. Plant Mol Biol. doi: 10.1007/s11103-011-9813-x.

Hamberger B, Ohnishi T, Hamberger B, Séguin A, and Bohlmann J. Evolution of diterpene metabolism: Sitka spruce CYP720B4 catalyses multiple oxidations in resin acid biosynthesis of conifer defense against insects. Plant Physiol. doi: 10.1104/pp.111.185843.

2010

Zulak KG, Dullat HK, Keeling CI, Lippert D, and Bohlmann J. Immunofluorescence localization of levopimaradiene/abietadiene synthase in methyl jasmonate treated stems of Sitka spruce (Picea sitchensis) shows activation of diterpenoid biosynthesis in cortical and developing traumatic resin ducts. Phytochemistry. doi: 10.1016/j.phytochem.2010.07.002.

Philippe RN, Ralph SG, Mansfield SD, and Bohlmann J. Transcriptome profiles of hybrid poplar (Populus trichocarpa x deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria). New Phytol. doi: 10.1111/j.1469-8137.2010.03392.x.

Robert JA and Bohlmann J. Behavioral and reproductive response of white pine weevil (Pissodes strobi) to resistant and susceptible Sitka spruce (Picea sitchensis). Insects. doi: 10.3390/insects1010003.

Robert JA, Madilao LL, White R. Yanchuk A, King J, and Bohlmann J. Terpenoid metabolite profiling in Sitka spruce identifies association of dehydroabietic acid, (+)-3-carene and terpinolene with resistance against white pine weevil. Botany. doi: 10.1139/B10-049.

Tsui CKM, Wang B, Khadempour L, Alamouti SM, Bohlmann J, Murray BW, and RC Hamelin. Rapid identification and detection of pine pathogenic fungi associated with mountain pine beetles by padlock probes. J Microbiol Meth. doi: 10.1016/j.mimet.2010.07.016.

Khadempour L, Massoumi Alamouti S, Hamelin R, Bohlmann J, and C Breuil. Target-specific PCR primers can detect and differentiate ophiostomatoid fungi from microbial communities associated with the mountain pine beetle Dendroctonus ponderosae. Fungal Biol-UK. doi: 10.1016/j.funbio.2010.08.001.

Hesse-Orce U, Diguistini S, Keeling CI, Wang Y, Docking R, Liao NY, Robertson G, Holt RA, Jones SJM, Bohlmann J, and C Breuil. Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera. BMC Genomics. doi: 10.1186/1471-2164-11-536.

Martin DM, Aubourg S, Schouwey MB, Daviet L, Schalk M, Toub O, Lund ST, and Bohlmann J. Functional annotation, genome organization and phylogeny of the grapevine (Vitis vinifera) terpene synthase gene family based on genome assembly, FLcDNA cloning, and enzyme assays. BMC Plant Biol. doi: 10.1186/1471-2229-10-226.

Zulak KG and Bohlmann J. Terpenoid biosynthesis and specialized vascular cells of conifer defense. J Integr Plant Biol. doi: 10.1111/j.1744-7909.2010.00910.x.

Keeling CI, Dullat HK, Yuen M, Ralph SG, Jancsik S, and Bohlmann J. Identification and functional characterization of monofunctional ent-copalyl diphosphate and ent-kaurene synthases in white spruce (Picea glauca) reveal different patterns for diterpene synthase evolution for primary and secondary metabolism in gymnosperm. Plant Physiol. doi: 10.1104/pp.109.151456.

Abbott E, Hall D, Hamberger B, and Bohlmann J. Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca). BMC Plant Biol. doi: 10.1186/1471-2229-10-106.

Wang Y, DiGuistini S, Wang T-CT, Bohlmann J, and C Breuil. Agrobacterium-meditated gene disruption using split-marker in Grosmannia clavigera, a mountain pine beetle associated pathogen. Current Genetics. doi: 10.1007/s00294-010-0294-2.

Albouyeh R, Farzaneh N, Bohlmann J, and K Ritland. Multivariate analysis of digital gene expression profiles identifies a xylem signature of the vascular tissue of white spruce (Picea glauca). Tree Genet & Genomes. doi: 10.1007/s11295-010-0275-0.

2009

Philippe RN, Ralph SG, Külheim C, Jancsik SI, and Bohlmann J. Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family. New Phytol. doi: 10.1111/j.1469-8137.2009.03028.x.

Tsui CKM, Feau N, Ritland CE, Massoumi Alamouti S, DiGuistini S, Bohlmann J, Breuil C, and RC Hamelin. Characterization of microsatellite loci in the fungus Grosmannia clavigera, a pine pathogen associated with the mountain pine beetle. Mol Ecol Resour. doi: 10.1111/j.1755-0998.2009.02717.x.

Lippert DN, Ralph SG, Phillips M, White R, Smith D, Hardie D, Gershenzon J, Ritland K, Borchers CH, and Bohlmann J. Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response. Proteomics. doi: 10.1002/pmic.200800252.

Lippert D, Yuen M, and Bohlmann J. Spruce Proteome DB: A resource for conifer proteomics research. Tree Genet & Genomes. doi: 10.1007/s11295-009-0220-2.

Diguistini S, Liao NY, Platt D, Robertson G, Seidel M, Chan SK, Docking TR, Birol I, Holt RA, Hirst M, Mardis E, Marra MA, Hamelin RC, Bohlmann J, Breuil C, and Jones SJ. De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data. Genome Biol. doi: 10.1186/gb-2009-10-9-r94.

Hamberger B, Hall D, Yuen M, Oddy C, Hamberger B, Keeling CI, Ritland C, Ritland K, and Bohlmann J. Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome. BMC Plant Biol. doi: 10.1186/1471-2229-9-106.

Bohlmann J and Gershenzon J. Old substrates for new enzymes of terpenoid biosynthesis. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.0905226106.

Dafoe NJ, Zamani A, Ekramoddoullah AK, Lippert D, Bohlmann J, and Constabel CP. Analysis of the poplar phloem proteome and its response to leaf wounding. J Proteome Res. doi: 10.1021/pr800968r.

Martin DM, Toub O, Chiang A, Lo BC, Ohse S, Lund ST, and Bohlmann J. The bouquet of grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) flowers arises from the biosynthesis of sesquiterpene volatiles in pollen grains. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.0901387106.

Liewlaksaneeyanawin C, Zhuang J, Tang M, Farzaneh N, Lueng G, Oddy C, Findlay S, Ritland CE, Bohlmann J, and Ritland K. Identification of COS markers in the Pinaceae. Tree Genet & Genomes. doi: 10.1007/s11295-008-0189-2.

Zulak KG, Lippert DN, Kuzyk MA, Domanski D, Chou T, Borchers CH, and Bohlmann J. Targeted proteomics using selected reaction monitoring (SRM) reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate treated Norway spruce (Picea abies). Plant J. doi: 10.1111/j.1365-313X.2009.04020.x.

2008

Godard KA, White R, and Bohlmann J. Monoterpene-induced molecular responses in Arabidopsis thaliana. Phytochemistry. doi: 10.1016/j.phytochem.2008.02.011.

Bohlmann J and Keeling CI. Terpenoid biomaterials. Plant J. doi: 10.1111/j.1365-313X.2008.03449.x.

Ehlting J, Chowrira SG, Mattheus N, Aeschliman DS, Arimura G, and Bohlmann J. Comparative transcriptome analysis of Arabidopsis thaliana infested by diamond back moth (Plutella xylostella) larvae reveals signatures of stress response, secondary metabolism, and signalling. BMC Genomics. doi: 10.1186/1471-2164-9-154.

Ralph SG, Chun HJ, Cooper D, Kirkpatrick R, Kolosova N, Gunter L, Tuskan GA, Douglas CJ, Holt RA, Jones SJ, Marra MA, and Bohlmann J. Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feeding. BMC Genomics. doi: 10.1186/1471-2164-9-57.

Keeling CI, Weisshaar S, Lin RP, and Bohlmann J. Functional plasticity of paralogous diterpene synthases involved in conifer defense. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.0709466105.

Holliday JA, Ralph SG, White R, Bohlmann J, and Aitken SN. Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis). New Phytol. doi: 10.1111/j.1469-8137.2007.02346.x.

Bohlmann J. Insect-induced terpenoid defenses in spruce. ln: “Induced Plant Resistance to Herbivory. A. Schaller (ed.) Springer Science. doi: 10.1007/978-1-4020-8182-8_8.

Keeling CI and Bohlmann J. Plant terpenoids. Wiley Encyclopedia of Chemical Biology. doi: 10.1002/9780470048672.wecb596.

Souza Cde A, Barbazuk B, Ralph SG, Bohlmann J, Hamberger B, and Douglas CJ. Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella. New Phytol. doi: 10.1111/j.1469-8137.2008.02534.x.

Jones CG, Keeling CI, Ghisalberti EL, Barbour EL, Plummer JA, and Bohlmann J. Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L. Arch Biochem Biophys. doi: 10.1016/j.abb.2008.05.008.

Ralph SG, Chun HJ, Kolosova N, Cooper D, Oddy C, Ritland CE, Kirkpatrick R, Moore R, Barber S, Holt RA, Jones SJ, Marra MA, Douglas CJ, Ritland K, and Bohlmann J. A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis). BMC Genomics. doi: 10.1186/1471-2164-9-484.

2007

Godard KA, Byun-McKay A, Levasseur C, Plant A, Seguin A, and Bohlmann J. Testing of a heterologous, wound- and insect-inducible promoter for functional genomics studies in conifer defense. Plant Cell Rep. doi: 10.1007/s00299-007-0417-5.

Peng FY, Reid KE, Liao N, Schlosser J, Lijavetzky D, Holt R, Martínez Zapater JM, Jones S, Marra M, Bohlmann J, and Lund ST. Generation of ESTs in Vitis vinifera wine grape (Cabernet Sauvignon) and table grape (Muscat Hamburg) and discovery of new candidate genes with potential roles in berry development. Gene. doi: 10.1016/j.gene.2007.07.016.

Phillips MA, Walter MH, Ralph SG, Dabrowska P, Luck K, Urós EM, Boland W, Strack D, Rodríguez-Concepción M, Bohlmann J, and Gershenzon J. Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies). Plant Mol Biol. doi: 10.1007/s11103-007-9212-5.

Behnke K, Ehlting B, Teuber M, Bauerfeind M, Louis S, Hänsch R, Polle A, Bohlmann J, and Schnitzler JP. Transgenic, non-isoprene emitting poplars don’t like it hot. Plant J. doi: 10.1111/j.1365-313X.2007.03157.x.

Ralph SG, Jancsik S, and Bohlmann J. Dirigent proteins in conifer defense II: Extended gene discovery, phylogeny, and constitutive and stress-induced gene expression in spruce (Picea spp.). Phytochemistry. doi: 10.1016/j.phytochem.2007.04.042.

Miranda M, Ralph SG, Mellway R, White R, Heath MC, Bohlmann J, and Constabel CP. The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to infection by Melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins. Mol Plant Microbe Interact. doi: 10.1094/MPMI-20-7-0816.

Kelleher CT, Chiu R, Shin H, Bosdet IE, Krzywinski MI, Fjell CD, Wilkin J, Yin T, DiFazio SP, Ali J, Asano JK, Chan S, Cloutier A, Girn N, Leach S, Lee D, Mathewson CA, Olson T, O’connor K, Prabhu AL, Smailus DE, Stott JM, Tsai M, Wye NH, Yang GS, Zhuang J, Holt RA, Putnam NH, Vrebalov J, Giovannoni JJ, Grimwood J, Schmutz J, Rokhsar D, Jones SJ, Marra MA, Tuskan GA, Bohlmann J, Ellis BE, Ritland K, Douglas CJ, and Schein JE. A physical map of the highly heterozygous Populus genome: integration with the genome sequence and genetic map and analysis of haplotype variation. Plant J. doi: 10.1111/j.1365-313X.2007.03112.x.

Huber DP, Erickson ML, Leutenegger CM, Bohlmann J, and Seybold SJ. Isolation and extreme sex-specific expression of cytochrome P450 genes in the bark beetle, Ips paraconfusus, following feeding on the phloem of host ponderosa pine, Pinus ponderosa. Insect Mol Biol. doi: 10.1111/j.1365-2583.2007.00731.x.

DiGuistini S, Ralph SG, Lim YW, Holt R, Jones S, Bohlmann J, and Breuil C. Generation and annotation of lodgepole pine and oleoresin-induced expressed sequences from the blue-stain fungus Ophiostoma clavigerum, a Mountain Pine Beetle-associated pathogen. FEMS Microbiol Lett. doi: 10.1111/j.1574-6968.2006.00565.x.

Friedmann M, Ralph SG, Aeschliman D, Zhuang J, Ritland K, Ellis BE, Bohlmann J, and Douglas CJ. Microarray gene expression profiling of developmental transitions in Sitka spruce (Picea sitchensis) apical shoots. J Exp Bot. doi: 10.1093/jxb/erl246.

Lippert D, Chowrira S, Ralph SG, Zhuang J, Aeschliman D, Ritland C, Ritland K, and Bohlmann J. Conifer defense against insects: proteome analysis of Sitka spruce (Picea sitchensis) bark induced by mechanical wounding or feeding by white pine weevils (Pissodes strobi). Proteomics. doi: 10.1002/pmic.200600525.

Ralph SG, Hudgins JW, Jancsik S, Franceschi VR, and Bohlmann J. Aminocyclopropane carboxylic acid synthase is a regulated step in ethylene-dependent induced conifer defense. Full-length cDNA cloning of a multigene family, differential constitutive, and wound- and insect-induced expression and cellular and subcellular localization in spruce and Douglas fir. Plant Physiol. doi: 10.1104/pp.106.089425.

Berube Y, Zhuang J, Ralph S, Rungis D, Bohlmann J, and Ritland K. Characterization of EST-SSRs in loblolly pine and spruce. Tree Genet & Genomes. doi: 10.1007/s11295-006-0061-1.

Philippe R.N. and Bohlmann J. Poplar defense against insect herbivores. Can J Bot. doi: 10.1139/B07-109.

2006

Hamberger B, and Bohlmann J. Cytochrome P450 mono-oxygenases in conifer genomes: discovery of members of the terpenoid oxygenase superfamily in spruce and pine. Biochem Soc Trans. doi: 10.1042/BST0341209.

Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, and Rokhsar D. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science. doi: 10.1126/science.1128691.

Hudgins JW, Ralph SG, Franceschi VR, and Bohlmann J. Ethylene in induced conifer defense response: FLcDNA cloning, wound-induced protein expression, and cellular and subcellular localization of 1-aminocyclopropane-carboxylic acid oxidase (ACO) in specialized resin duct cells and polyphenolic phloem parechny. Planta. doi: 10.1007/s004250000274.

Seybold SJ, Huber DPW, Lee LC, and Bohlmann J. Pine monoterpenes and pine bark beetles: A marriage of convenience for defense and chemical communication. Phytochemistry Rev. doi: 10.1007/s11101-006-9002-8.

Phillips M, Bohlmann J, and J Gershenzon. Molecular regulation of induced terpenoid biosynthesis in conifers. Phytochemistry Rev. doi: 10.1007/s11101-006-0001-6.

Ralph SG, Yueh H, Friedmann M, Aeschliman D, Zeznik JA, Nelson CC, Butterfield YS, Kirkpatrick R, Liu J, Jones SJ, Marra MA, Douglas CJ, Ritland K, and Bohlmann J. Conifer defence against insects: microarray gene expression profiling of Sitka spruce (Picea sitchensis) induced by mechanical wounding or feeding by spruce budworms (Choristoneura occidentalis) or white pine weevils (Pissodes strobi) reveals large-scale changes of the host transcriptome. Plant Cell Environ. doi: 10.1111/j.1365-3040.2006.01532.x.

Ro DK, and Bohlmann J. Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): Functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1). Phytochemistry. doi: 10.1016/j.phytochem.2006.01.011.

Ro DK, Ehlting J, Keeling CI, Lin R, Mattheus N, and Bohlmann J. Microarray expression profiling and functional characterization of AtTPS genes: duplicated Arabidopsis thaliana sesquiterpene synthase genes At4g13280 and At4g13300 encode root-specific and wound-inducible (Z)-gamma-bisabolene synthases. Arch Biochem Biophys. doi: 10.1016/j.abb.2005.09.019.

Ralph S, Oddy C, Cooper D, Yueh H, Jancsik S, Kolosova N, Philippe RN, Aeschliman D, White R, Huber D, Ritland CE, Benoit F, Rigby T, Nantel A, Butterfield YS, Kirkpatrick R, Chun E, Liu J, Palmquist D, Wynhoven B, Stott J, Yang G, Barber S, Holt RA, Siddiqui A, Jones SJ, Marra MA, Ellis BE, Douglas CJ, Ritland K, and Bohlmann J. Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar. Mol Ecol. doi: 10.1111/j.1365-294X.2006.02824.x.

Gilchrist EJ, Haughn GW, Ying CC, Otto SP, Zhuang J, Cheung D, Hamberger B, Aboutorabi F, Kalynyak T, Johnson L, Bohlmann J, Ellis BE, Douglas CJ, and Cronk QC. Use of Ecotilling as an efficient SNP discovery tool to survey genetic variation in wild populations of Populus trichocarpa. Mol Ecol. doi: 10.1111/j.1365-294X.2006.02885.x.

Byun-McKay A, Godard KA, Toudefallah M, Martin DM, Alfaro R, King J, Bohlmann J, and Plant AL. Wound-induced terpene synthase gene expression in Sitka spruce that exhibit resistance or susceptibility to attack by the white pine weevil. Plant Physiol. doi: 10.1104/pp.105.071803.

Lund ST and Bohlmann J. The molecular basis for wine grape quality—a volatile subject. Science. doi: 10.1126/science.1118962.

Ralph S, Park JY, Bohlmann J, and Mansfield SD. Dirigent proteins in conifer defense: gene discovery, phylogeny, and differential wound- and insect-induced expression of a family of DIR and DIR-like genes in spruce (Picea spp.). Plant Mol Biol. doi: 10.1007/s11103-005-2226-y.

Keeling CI, and Bohlmann J. Genes, enzymes and chemicals of terpenoid diversity in the constitutive and induced defence of conifers against insects and pathogens. New Phytol. doi: 10.1111/j.1469-8137.2006.01716.x.

Ritland K, Ralph S, Lippert D, Rungis D, and Bohlmann J. A new direction in conifer genomics. In: Landscapes, Genomics and Transgenic Conifer Forests. Claire Williams (ed.) Springer Publishers, Dordrecht Netherlands. pp. 75–84.

2005

Huber DP, Philippe RN, Madilao LL, Sturrock RN, and Bohlmann J. Changes in anatomy and terpene chemistry in roots of Douglas-fir seedlings following treatment with methyl jasmonate. Tree Physiol. 25, 1075–83.

Ehlting J, Mattheus N, Aeschliman DS, Li E, Hamberger B, Cullis IF, Zhuang J, Kaneda M, Mansfield SD, Samuels L, Ritland K, Ellis BE, Bohlmann J, and Douglas CJ. Global transcript profiling of primary stems from Arabidopsis thaliana identifies candidate genes for missing links in lignin biosynthesis and transcriptional regulators of fiber differentiation. Plant J. doi: 10.1111/j.1365-313X.2005.02403.x.

Huber DP, Philippe RN, Godard KA, Sturrock RN, and Bohlmann J. Characterization of four terpene synthase cDNAs from methyl jasmonate-induced Douglas-fir, Pseudotsuga menziesii. Phytochemistry. doi: 10.1016/j.phytochem.2005.04.030.

Ro DK, Arimura G, Lau SY, Piers E, and Bohlmann J. Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.0500825102.

Lippert D, Zhuang J, Ralph S, Ellis DE, Gilbert M, Olafson R, Ritland K, Ellis B, Douglas CJ, and Bohlmann J. Proteome analysis of early somatic embryogenesis in Picea glauca. Proteomics. doi: 10.1002/pmic.200400986.

Miller B, Madilao LL, Ralph S, and Bohlmann J. Insect-induced conifer defense. White pine weevil and methyl jasmonate induce traumatic resinosis, de novo formed volatile emissions, and accumulation of terpenoid synthase and putative octadecanoid pathway transcripts in Sitka spruce. Plant Physiol. doi: 10.1104/pp.104.050187.

Martin D and Bohlmann J. Molecular biochemistry and genomics of terpenoid defenses in conifers. Recent Adv Phytochem. doi: 10.1016/S0079-9920(05)80003-6.

Rungis D, Hamberger B, Berube Y, Wilkin J, Bohlmann J, and K Ritland. Efficient genetic mapping of single nucleotide polymorphisms based upon DNA mismatch digestion. Mol Breeding. doi: 10.1007/s11032-005-3424-7.

2004

Huber DP, Ralph S, and Bohlmann J. Genomic hardwiring and phenotypic plasticity of terpenoid-based defenses in conifers. J Chem Ecol. doi: https://doi.org/10.1007/s10886-004-7942-2.

Rungis D, Berube Y, Zhang J, Ralph S, Ritland CE, Ellis BE, Douglas C, Bohlmann J, and Ritland K. Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags. Theor Appl Genet. doi: 10.1007/s00122-004-1742-5.

Lucker J, Bowen P, and Bohlmann J. Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries. Phytochemistry. doi: 10.1016/j.phytochem.2004.08.017.

Chen F, Ro DK, Petri J, Gershenzon J, and Bohlmann J, Pichersky E, Tholl D. Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole. Plant Physiol. doi: 10.1104/pp.104.044388.

Arimura G, Ozawa R, Kugimiya S, Takabayashi J, and Bohlmann J. Herbivore-induced defense response in a model legume. Two-spotted spider mites induce emission of (E)-beta-ocimene and transcript accumulation of (E)-beta-ocimene synthase in Lotus japonicus. Plant Physiol. doi: 10.1104/pp.104.042929.

Martin DM, Fäldt J, and Bohlmann J. Functional characterization of nine Norway Spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily. Plant Physiol. doi: 10.1104/pp.104.042028.

Reiling KK, Yoshikuni Y, Martin VJ, Newman J, Bohlmann J, and Keasling JD. Mono and diterpene production in Escherichia coli. Biotechnol Bioeng. doi: 10.1002/bit.20128.

Kolosova N, Miller B, Ralph S, Ellis BE, Douglas C, Ritland K, and Bohlmann J. Isolation of high-quality RNA from gymnosperm and angiosperm trees. Biotechniques. doi: 10.2144/04365ST06

Martin DM and Bohlmann J. Identification of Vitis vinifera (-)-alpha-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase. Phytochemistry. doi: 10.1016/j.phytochem.2004.03.018.

Arimura G, Huber DP, and Bohlmann J. Forest tent caterpillars (Malacosoma disstria) induce local and systemic diurnal emissions of terpenoid volatiles in hybrid poplar (Populus trichocarpa x deltoides): cDNA cloning, functional characterization, and patterns of gene expression of (-)-germacrene D synthase, PtdTPS1. Plant J. doi: 10.1111/j.1365-313X.2003.01987.x.

Huber DPW and Bohlmann J. Terpene synthases and the mediation of plant-insect ecological interactions by terpenoids: a mini-review. In: Plant Adaptation: Molecular Genetics and Ecology. Q.C.B. Cronck, J. Whitton, R.H. Ree, I.E.P. Taylor. NRC Research Press, Ottawa, Ontario. doi: 10.1139/9780660193366.

2003

McKay SA, Hunter WL, Godard KA, Wang SX, Martin DM, Bohlmann J, and Plant AL. Insect attack and wounding induce traumatic resin duct development and gene expression of (-)-pinene synthase in Sitka spruce. Plant Physiol. doi: 10.1104/pp.103.022723.

Martin DM, Gershenzon J, and Bohlmann J. Induction of volatile terpene biosynthesis and diurnal emission by methyl jasmonate in foliage of Norway spruce. Plant Physiol. doi: 10.1104/pp.103.021196.

Dudareva N, Martin D, Kish CM, Kolosova N, Gorenstein N, Fäldt J, Miller B, and Bohlmann J. (E)-beta-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon: function and expression of three terpene synthase genes of a new terpene synthase subfamily. Plant Cell. doi: 10.1105/tpc.011015.

Martin D, Bohlmann J, Gershenzon J, Francke W, and Seybold SJ. A novel sex-specific and inducible monoterpene synthase activity associated with a pine bark beetle, the pine engraver, Ips pini. Naturwissenschaften. doi: 10.1007/s00114-003-0410-y.

Fäldt J, Arimura G, Gershenzon J, Takabayashi J, and Bohlmann J. Functional identification of AtTPS03 as (E)-beta-ocimene synthase: a monoterpene synthase catalyzing jasmonate- and wound-induced volatile formation in Arabidopsis thaliana. Planta. doi: 10.1007/s00425-002-0924-0.

Fäldt J, Martin D, Miller B, Rawat S, and Bohlmann J. Traumatic resin defense in Norway spruce (Picea abies): methyl jasmonate-induced terpene synthase gene expression, and cDNA cloning and functional characterization of (+)-3-carene synthase. Plant Mol Biol. doi: 10.1023/A:1020714403780.

2002

Aubourg S, Lecharny A, and Bohlmann J. Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana. Mol Genet Genomics. doi: 10.1007/s00438-002-0709-y.

Martin D, Tholl D, Gershenzon J, and Bohlmann J. Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant Physiol. doi: 10.1104/pp.011001.

Bohlmann J, Stauber EJ, Krock B, Oldham NJ, Gershenzon J, and Baldwin IT. Gene expression of 5-epi-aristolochene synthase and formation of capsidiol in roots of Nicotiana attenuata and N. sylvestris. Phytochemistry. doi: 10.1016/S0031-9422(02)00080-8.

Alfaro RI, Borden JH, King JN, Tomlin ES, McIntosh RL, and Bohlmann J. Mechanisms of Resistance in Conifers against shoot infesting insects. In:Mechanisms and Deployment of Resistance in Trees to Insects. M.R. Wagner, K.M. Clancy, F. Lieutier, and T.D. Paine”. Kluwer Academic Press, Dordrecht, the Netherlands. doi: 10.1007/0-306-47596-0_4.

2000

Bohlmann J, Gershenzon J and S Aubourg. Biochemical, molecular genetic and evolutionary aspects of defense-related terpenoid metabolism in conifers. In: Evolution of metabolic pathways, Romeo J.T. et al. (eds) Recent Adv Phytochem. 34, 109–150.

Bohlmann J, Martin D, Oldham NJ and J Gershenzon. Terpenoid secondary metabolism in Arabidopsis thaliana: cDNA cloning, characterization and functional expression of a myrcene / (E)-b-ocimene synthase. Arch Biochem Biophys. doi: 10.1006/abbi.1999.1669.

Seybold SJ, Bohlmann J and KF Raffa. The biosynthesis of coniferophagous bark beetle pheromones and conifer isoprenoids: Evolutionary perspective and synthesis. Can Entomol. doi: 10.4039/Ent132697-6.

1999

Bohlmann J and R Croteau. Diversity and variability of terpenoid defenses in conifers: molecular genetics, biochemistry and evolution of the terpene synthase gene family in grand fir (Abies grandis). Novart Fdn Symp. doi: 10.1002/9780470515679.ch9.

Bohlmann J, Phillips M, Ramachandiran V, Katoh S and R Croteau. cDNA cloning, characterization, and functional expression of four new monoterpene synthases of the TPSd gene family from grand fir (Abies grandis). Arch Biochem Biophys. doi: 10.1006/abbi.1999.1332.

1998

Bohlmann J, Crock J, Jetter R and R Croteau. Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-a-bisabolene synthase from grand fir (Abies grandis). Proc Natl Acad Sci U S A. doi: 10.1073/pnas.95.12.6756.

Bohlmann J, Meyer-Gauen G and R Croteau. Plant terpenoid synthases: molecular biology and phylogenetic analysis. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.95.8.4126.

Steele CL, Katoh S, Bohlmann J and R Croteau. Regulation of oleoresinosis in grand fir (Abies grandis). Differential transcriptional control of monoterpene, sesquiterpene and diterpene synthase genes in response to wounding. Plant Physiol. doi: 10.1104/pp.116.4.1497.

Steele CL, Crock J, Bohlmann J and R Croteau. Sesquiterpene synthases from grand fir (Abies grandis). Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of d-selinene synthase and g-humulene synthase. J Biol Chem. doi: 10.1074/jbc.273.4.2078.

1997

Bohlmann J, Steele CL and R Croteau. Monoterpene synthases from grand fir (Abies grandis): cDNA isolation, characterization and functional expression of myrcene synthase, (-)-4S-limonene synthase, and (-)-1S:5S-pinene synthase. J Biol Chem. doi: 10.1074/jbc.272.35.21784.

1996

Bohlmann J, Lins T, Martin W and U Eilert. Anthranilate synthase from Ruta graveolens: Duplicated ASa genes encode tryptophan-sensitive and tryptophan-insensitive isoenzymes specific to amino acid and alkaloid biosynthesis. Plant Physiol. doi: 10.1104/pp.111.2.507.

1995

Bohlmann J, DeLuca V, Eilert U and W Martin. Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: Modes of expression and properties of native and recombinant enzymes. Plant J. doi: 10.1046/j.1365-313X.1995.7030491.x.

Bohlmann J, Gibraltarskaya E and U Eilert. Elicitor induction of furanocoumarin biosynthetic pathway in cell cultures of Ruta graveolens L. Plant Cell Tiss Org. doi: 10.1007/BF00052171.

1994

Bohlmann J and U Eilert. Elicitor induced secondary metabolism in Ruta graveolens L.: Role of chorismate utilizing enzymes. Plant Cell Tiss Org. doi: 10.1007/978-94-011-0237-7_13.