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Publications from the Brumer lab

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Peer-reviewed articles and book chapters (* indicates corresponding author).  Patent applications are listed separately below.

Dr. Brumer also coordinates and actively contributes to CAZypedia - The Encyclopedia of Carbohydrate-Active Enzymes (see also article in Glycobiology, DOI:10.1093/glycob/cwx089).  In addition, work in the group has produced the MediaWiki software extension BiblioPlus.


125. The CAZypedia Consortium (2017) Ten years of CAZypedia: A living encyclopedia of carbohydrate-active enzymes. Glycobiology, 28, 3-8. DOI:10.1093/glycob/cwx089


124. Nelson, C.E., Attia, M.A., Rogowski, A., Morland, C., Brumer, H., Gardner, J.G.* (2017) Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification. Environ. Microbiol., 19, 5025-5039. DOI:10.1111/1462-2920.13959

123. Tamura, K., Hemsworth, G.R., Déjean, G., Rogers, T.E., Pudlo, N.A., Urs, K., Jain, N., Davies, G.J., Martens, E.C., Brumer, H.* (2017) Molecular mechanism by which prominent human gut Bacteroidetes utilize mixed-linkage beta-glucans, major health-promoting cereal polysaccharides, Cell Reports, 21, 417-430. DOI:10.1016/j.celrep.2017.09.049

122. Grondin, J.M., Tamura, K., Déjean, G., Abbott, D.W.*, Brumer, H.* (2017) Polysaccharide Utilization Loci: Fuelling microbial communities. J. Bacteriol., 99, e00860-16. DOI:10.1128/JB.00860-16

121. McGregor, N., Arnal, G., Brumer, H.* (2017) Quantitative kinetic characterization of glycoside hydrolases using high-performance anion-exchange chromatography (HPAEC), Chapter 2 in Protein-Carbohydrate Interactions: Methods and Protocols, Abbott, D.W. and Lammerts van Bueren, A. (eds.), Methods in Molecular Biology, 1588, 15-25. DOI:10.1007/978-1-4939-6899-2_2

120. Arnal, G., Attia, M.A., Asohan, J., Brumer, H.* (2017) A low-volume, parallel copper-bicinchoninic acid (BCA) assay for glycoside hydrolases, Chapter 1 in Protein-Carbohydrate Interactions: Methods and Protocols, Abbott, D.W. and Lammerts van Bueren, A. (eds.), Methods in Molecular Biology, 1588, 3-14. DOI:10.1007/978-1-4939-6899-2_1

119. Fanuel, M., Garajova, S., Ropartz, D., McGregor, N., Brumer, H., Rogniaux, H.,* Berrin, J.G.* (2017) The Podospora anserina lytic polysaccharide monooxygenase PaLPMO9H catalyzes oxidative cleavage of diverse plant cell wall matrix glycans. Biotechnol Biofuels, 10, 63. DOI:10.1186/s13068-017-0749-5

118. McGregor, N., Yin, V., Tung, C.-C., van Petegem, F., Brumer, H.* (2017) Crystallographic insight into the evolutionary origins of xyloglucan endo-transglycosylases and endo-hydrolases. Plant J., 89, 651-670. DOI:10.1111/tpj.13421 (Featured Article, cover image: DOI:10.1111/tpj.13356)

117. Larsbrink, J, Tuveng, T.R., Pope, P.B., Bulone, V., Eijsink, V.G., Brumer, H, McKee, L.S.* (2017) Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J. Proteomics, 156, 63–74. DOI:10.1016/j.jprot.2017.01.003

116. Larsbrink, J, Tuveng, T.R., Pope, P.B., Bulone, V., Eijsink, V.G., Brumer, H, McKee, L.S.* (2017) Proteomic data on enzyme secretion and activity in the bacterium Chitinophaga pinensis, Data in Brief, 11, 484–490. DOI:10.1016/j.dib.2017.02.032

115. Robb, M., Hobbs, J.K., Woodiga, S.A., Shapiro-Ward, S., Suits, M.D.L., McGregor, N., Brumer, H., Yesilkaya, H., King, S.J., Boraston, A.B.* (2017) Molecular characterization of N-glycan degradation and transport in Streptococcus pneumoniae and its contribution to virulence, PLoS Pathog., 13, e1006090. DOI:10.1371/journal.ppat.1006090


114. Benselfelt, T., Cranston, E.D., Ondaral, S., Johansson, E., Brumer, H., Rutland, M.W., Wågberg, L.* (2016) Adsorption of xyloglucan onto cellulose surfaces of different morphologies: An entropy-driven process. Biomacromolecules, 17, 2801–2811. DOI:10.1021/acs.biomac.6b00561

113. Hemsworth, G.R., Thompson, A.J., Stepper, J., Sobala, Ł.F., Coyle, T., Larsbrink, J., Spadiut, O., Goddard-Borger, E.D., Stubbs, K.A., Brumer, H.*, Davies, G.J.* (2016) Structural dissection of a complex Bacteroides ovatus gene locus conferring xyloglucan metabolism in the human gut. Open Biol., 6, Article number: 160142. DOI:10.1098/rsob.160142

112. Attia, M.A., Brumer, H.* (2016) Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan. Curr. Opin. Struct. Biol., 40, 43-53. DOI:10.1016/

111. Attia, M., Stepper, J., Davies, G.J., Brumer, H.* (2016) Functional and structural characterization of a potent GH74 endo-xyloglucanase from the soil saprophyte Cellvibrio japonicus unravels the first step of xyloglucan degradation. FEBS J., 283, 1701-1719. DOI:10.1111/febs.13696

110. Tauzin, A.S., Kwiatkowski, K.J., Orlovsky, N.I., Smith, C.J., Creagh, A.L., Haynes, C.A., Wawrzak, Z., Brumer, H.*, Koropatkin, N.M.* (2016) Molecular dissection of xyloglucan recognition in a prominent human gut symbiont. mBio, 7, e02134-15. DOI:10.1128/mBio.02134-15

109. Derikvand, F., Yin, D.T., Barrett, R., Brumer, H.* (2016) Cellulose-based biosensors for esterase detection. Anal. Chem., 88, 2989–2993. DOI:10.1021/acs.analchem.5b04661

108. Hemsworth, G.R.,* Déjean, G., Davies, G.J., Brumer, H. (2016) Learning from microbial strategies for polysaccharide degradation. Biochem. Soc. Trans., 44, 94-108. DOI:10.1042/BST20150180

107. McGregor, N., Morar, M., Fenger, T.H., Stogios, P., Lenfant, N., Yin, V., Xu, X., Evdokimova, E., Cui, H., Henrissat, B., Savchenko, A.,* Brumer, H.* (2016) Structure-function analysis of a mixed-linkage beta-glucanase/xyloglucanase from key ruminal Bacteroidetes Prevotella bryantii B14. J. Biol. Chem., 291, 1175-1197. DOI:10.1074/jbc.M115.691659


106. Yin, D.T., Urresti, S., Lafond, M., Johnston, E.M., Derikvand, F., Ciano, L., Berrin, J.G., Henrissat, B., Walton, P.H., Davies, G.J., Brumer, H.* (2015) Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family. Nat. Commun., 6,
Article number: 10197. DOI:10.1038/ncomms10197

105. Gerttula, S., Zinkgraf, M., Muday, G., Lewis, D., Ibatullin, F.M., Brumer, H., Hart, F., Mansfield, S.D., Filkov, V., Groover, A.* (2015) Transcriptional and hormonal regulation of gravitropism of woody stems in Populus. Plant Cell, 27, 2800–2813. DOI:10.1105/tpc.15.00531 (Selected for the issue cover image. Comment in DOI:10.1105/tpc.15.00824)

104. McKee, L.S., Brumer, H.* (2015) Growth of Chitinophaga pinensis on plant cell wall glycans and characterisation of a Glycoside Hydrolase Family 27 β-L-arabinopyranosidase implicated in arabinogalactan utilisation. PLoS ONE, 10(10), e0139932. DOI:10.1371/journal.pone.0139932

103. Coyle, T., Brumer, H., Stubbs, K.A.* (2015) An improved preparation of some aryl alpha-L-arabinofuranosides for use as chromogenic substrates for alpha-L-arabinofuranosidases. Can. J. Chem., 93, 1176-1180. DOI:10.1139/cjc-2015-0227

102. Fenger, T.H., Brumer, H.* (2015) Synthesis and analysis of specific covalent inhibitors of endo-xyloglucanases. ChemBioChem., 16, 575–583.  DOI:10.1002/cbic.201402663


101. Oksanen, A.,* Retulainen, E., Brumer, H. (2014) Bulky paper with good strength and smoothness? Certainly! Nord Pulp Pap. Res. J., 29, 725-731. DOI:10.3183/NPPRJ-2014-29-04-p725-731

100. Banasiak, A., Ibatullin, F.M., Brumer, H., Mellerowicz, E.J.* (2014) Glycoside hydrolase activities in cell walls of sclerenchyma cells in the inflorescence stems of Arabidopsis thaliana visualized in situ. Plants, 3, 513-525. DOI:10.3390/plants3040513

99. Larsbrink, J., Thompson, A.J., Lundqvist, M., Gardner, J.G., Davies, G.J., Brumer, H.* (2014) A complex gene locus enables xyloglucan utilization in the model saprophyte Cellvibrio japonicus. Mol. Microbiol., 94, 418–433. DOI:10.1111/mmi.12776

98. Brumer, H.,* Gilbert, H.* (2014) Editorial Overview: Carbohydrate-protein interactions: The future is taking shape. Curr. Opin. Struct. Biol., 28, v–vii. DOI:10.1016/

97. Martens, E.C.,* Kelly, A.G., Tauzin, A.S., Brumer, H.* (2014) The devil lies in the details: How variations in polysaccharide fine-structure impact the physiology and evolution of gut microbes. J. Mol. Biol., 426, 3851–3865. DOI:10.1016/j.jmb.2014.06.022

96. Anasontzis, G.E.,* Penã, M.S., Spadiut, O., Brumer, H., Olsson, L. (2014) Effects of temperature and glycerol- and methanol- feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris. Biotechnol. Progress, 30, 728-735. DOI:10.1002/btpr.1878

95. Larsbrink, J., Rogers, T.E., Hemsworth, G.R., McKee, L.S., Tauzin, A.S., Spadiut, O., Klinter, S., Pudlo, N.A., Urs, K., Koropatkin, N.M., Creagh, A.L., Haynes, C.A., Kelly, A.G., Nilsson Cederholm, S., Davies, G.J.*, Martens, E.C.*, Brumer, H.* (2014) A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes. Nature, 506, 498–502. DOI:10.1038/nature12907 (Press releases: UBC, MSL, University of York, KTH; Spotlight: Trends Biochem. Sci.; Recommended: F1000Prime; Headline: UniProt release 2014_03)

94. Song, Y., Gyarmati, P., Araújo, A.C., Lundeberg, J., Brumer, H., Ståhl, P.L.* (2014) Visual Detection of DNA on Paper Chips. Anal. Chem.86, 1575-1582. DOI:10.1021/ac403196b

93. Wang, Y., Vilaplana, F., Brumer, H., Aspeborg, H.* (2014) Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5_7) mannanase from Arabidopsis thaliana. Planta239, 653-665. DOI:10.1007/s00425-013-2005-y

92. Leppänen, A.-S.,* Xu, C., Parikka, K., Eklund, P., Sjöholm, R., Brumer, H., Tenkanen, M., Willför, S. (2014) Targeted allylation and propargylation of galactose-containing polysaccharides in water. Carbohydr. Polym., 100, 46-54. DOI:10.1016/j.carbpol.2012.11.053


91. Eklöf, J.M., Shojania, S., Okon, M., McIntosh, L.P., Brumer, H.* (2013) Structure-function analysis of a broad-specificity Populus trichocarpa endo-beta-glucanase reveals an evolutionary link between bacterial licheninases and plant XTH gene products. J. Biol. Chem., 288, 15786-15799. DOI:10.1074/jbc.M113.462887

90. Thongpoo, P., McKee, L.S., Araújo, A.C., Kongsaeree, P.T., Brumer, H.* (2013) Identification of the acid/base catalyst of a glycoside hydrolase family 3 (GH3) beta-glucosidase from Aspergillus niger ASKU28. Biochim. Biophys. Acta - Gen. Subj., 1830, 2739–2749. DOI:10.1016/j.bbagen.2012.11.014

89. Stepper, J., Dabin, J., Eklöf, J.M., Thongpoo, P., Kongsaeree, P., Taylor, E., Turkenburg, J.P., Brumer, H., Davies, G.J.* (2013) Structure and Activity of the Streptococcus pyogenes family GH1 6-phospho beta-D-glycosidase, SPy1599. Acta Crystallogr. D Biol. Crystallogr., D69, 16-23. DOI:10.1107/S0907444912041005

88. Kaewthai, N., Gendre, D., Eklöf, J.M., Ibatullin, F.M., Ezcurra, I, Bhalerao, R.P., Brumer, H.* (2013) Group III-A XTH genes of Arabidopsis thaliana encode predominant xyloglucan endo-hydrolases that are dispensable for normal growth. Plant Physiol., 161, 440-454.  DOI:10.1104/pp.112.207308


87. Larsbrink, J., Izumi, A., Hemsworth, G.R., Davies, G.J.,* Brumer, H.* (2012) Structural enzymology of Cellvibrio japonicus Agd31B reveals α-transglucosylase activity in glycoside hydrolase family 31. J. Biol. Chem., 287, 43288–43299. DOI:10.1074/jbc.M112.416511

86. Aspeborg, H., Coutinho, P.M., Wang, Y., Brumer, H., Henrissat, B.* (2012) Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5). BMC Evolutionary Biology,12, 186. DOI:10.1186/1471-2148-12-186

85. Parikka, K.,* Leppänen, A.S., Xu, C., Pitkänen, L., Eronen, P., Österberg, M., Brumer, H., Willför, S., Tenkanen, M. (2012) Functional and anionic cellulose-interacting polymers by selective chemo-enzymatic carboxylation of galactose-containing polysaccharides. Biomacromolecules, 13, 2418-2428. DOI:10.1021/bm300679a

84. Silipo, A.,* Larsbrink, L., Marchetti, R., Lanzetta, R., Brumer, H.,* Molinaro,A. (2012) NMR analysis reveals extensive binding interactions of complex xyloglucan oligosaccharides with the Cellvibrio japonicus Glycoside Hydrolase Family 31 alpha-xylosidase. Chemistry, 18, 13395–13404. DOI:10.1002/chem.201200488

83. Araújo, A.C., Nakhai, A., Ruda, M., Slättegård, R., Gatenholm, P., Brumer, H.* (2012) A general route to xyloglucan-peptide conjugates for the activation of cellulose surfaces. Carbohydr. Res., 354, 116-120. DOI:10.1016/j.carres.2012.03.038

82. Eklöf, J.M., Ruda, M., Brumer, H.* (2012) Distinguishing xyloglucanase activity in endo-beta(1-4)glucanases. Method. Enzymol., Vol. 510, Ch. 6, pp. 97-120. DOI:10.1016/B978-0-12-415931-0.00006-9

81. Araújo, A.C., Song, Y., Lundeberg, J., Ståhl, P.L., Brumer, H.* (2012) Activated paper surfaces for the rapid hybridization of DNA through capillary transport. Anal. Chem., 84, 3311–3317. DOI:10.1021/ac300025v (Correction in Anal. Chem., 2013, 85, 11676. DOI: 10.1021/ac403673s)

80.  Bi, R., Spadiut, O., Lawoko, M., Brumer, H., Henriksson, G. (2012) Isolation and identification of microorganisms from soil able to live on lignin as a carbon source and to produce enzymes which cleave the beta-O-4 bond in a lignin model compound. Cellulose Chem. Technol., 46, 227-242. Online access: Article, Issue.

79. Xu, C., Spadiut, O., Araújo, A.C., Nakhai, A., Brumer, H.* (2012) Chemo-enzymatic assembly of clickable cellulose surfaces via multivalent polysaccharides. ChemSusChem, 5, 661–665. DOI:10.1002/cssc.201100522


78. Zhang, Q., Brumer, H., Ågren, H., Tu, Y.* (2011) The adsorption of xyloglucan on cellulose: Effects of explicit water and side chain variation. Carbohydr. Res., 346, 2595-2602. DOI:10.1016/j.carres.2011.09.007

77. Ariza, A., Eklöf, J.M., Spadiut, O., Offen, W.A., Roberts, S.M., Besenmatter, W., Friis, E.P., Skjot, M., Wilson, K.S.*, Brumer, H.*, Davies, G.J.* (2011) Structure and activity of a Paenibacillus polymyxa xyloglucanase from glycoside hydrolase family 44. J. Biol. Chem., 286, 33890-33900. DOI:10.1074/jbc.M111.262345

76. Spadiut, O., Ibatullin, F.M., Peart, J., Gullfot, F., Martinez-Fleites, C., Ruda, M.C., Xu, C., Sundqvist, G., Davies, G.J., Brumer, H.* (2011) Building custom polysaccharides in vitro with an efficient, broad-specificity xyloglucan glycosynthase and a fucosyltransferase. J. Am. Chem. Soc., 133, 10892-10900. DOI:10.1021/ja202788q (Showcased in "The Chemistry-Glycobiology Frontier" issue of JACS Select)

75. Mark, P., Zhang, Q., Czjzek, M., Brumer, H., Ågren, H.* (2011) Molecular dynamics simulations of a branched tetradecasaccharide substrate in the active site of a xyloglucan endo-transglycosylase. Mol. Simulat., 37, 1001-1013. DOI:10.1080/08927022.2011.566605

74. Larsbrink, J., Izumi, A., Ibatullin, F.M., Nakhai, A., Gilbert, H.J., Davies, G.J., Brumer, H.* (2011) Structural and enzymatic characterisation of a Glycoside Hydrolase Family 31 alpha‑xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification. Biochem. J., 436, 567-580. DOI: 10.1042/BJ20110299

73. Oksanen, A.,* Rantanen, T., Retulainen, E., Salminen, K., Brumer, H. (2011) Improving Wet Web Runnability and Paper Quality by an Uncharged Polysaccharide. Journal of Biobased Materials and Bioenergy, 5, 187-191. DOI: 10.1166/jbmb.2011.1144

72. Cartmell, A., McKee, L., Pena, M.J., Larsbrink, J., Brumer, H., Kaneko, S., Ichinose, H., Lewis, R.J., Vikso-Nielsen, A., Gilbert, H.J.,* Marles-Wright, J. (2011) The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases. J. Biol. Chem., 286, 15483-15495. DOI: 10.1074/jbc.M110.215962

71. Fink, H., Ahrenstedt, L., Bodin, A., Brumer, H., Gatenholm, P., Krettek, A., Risberg, B. (2011) Bacterial cellulose modified with xyloglucan bearing the adhesion peptide RGD promotes endothelial cell adhesion and metabolism—a promising modification for vascular grafts. J. Tissue Eng. Regen. Med., 5, 454-463. DOI: 10.1002/term.334

70. Maris, A., Kaewthai, N., Eklöf, J.M., Miller, J.G., Brumer, H., Fry, S.C., Verbelen, J.-P., Vissenberg, K.* (2011) Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis thaliana. J. Exp. Bot., 62, 261-271. DOI:10.1093/jxb/erq263.

69. Nishikubo, N., Takahashi, J., Roos, A.A., Derba-Maceluch, M., Piens, K., Brumer, H., Teeri, T.T., Stålbrand, H., and Mellerowicz, E.J.* (2011) Xyloglucan endo-transglycosylase-mediated xyloglucan rearrangements in developing wood of hybrid aspen (Populus tremula x tremuloides). Plant Physiol., 155, 399-413. DOI: 10.1104/pp.110.166934


68. Lombard, V., Bernard, T., Rancurel, C., Brumer, H., Coutinho, P.M., and Henrissat, B.* (2010) A hierarchical classification of polysaccharide lyases for glycogenomics. Biochem. J., 432, 437–444. DOI: 10.1042/BJ20101185

67. Spadiut, O., Olsson, L., Brumer, H.* (2010) A comparative summary of expression systems for the recombinant production of galactose oxidase. Microb. Cell Fact., 9:68. DOI: 10.1186/1475-2859-9-68

66. Eklöf, J.M., Brumer, H.* (2010) The XTH gene family: An update on enzyme structure, function, and phylogeny in xyloglucan remodelling. Plant Physiol., 153, 456-466. DOI:10.1104/pp.110.156844.

65. Kaewthai, N., Harvey, A.J., Hrmova, M., Brumer, H.*, Ezcurra, I., Teeri, T.T., Fincher, G.B. (2010) Heterologous expression of diverse barley XTH genes in the yeast Pichia pastoris. Plant Biotechnology, 27, 251-258. DOI: 10.5511/plantbiotechnology.27.251 

64. Brumer, H. (2010) "Carbohydrases" in The Encyclopedia of Catalysis, 2nd Edition, István T. Horváth, ed., John Wiley and Sons, New York (Online 5 March). DOI: 10.1002/0471227617.eoc037

63. Brumer, H. (2010) "Enzymatic functionalization of cellulosic fibres: xyloglucan as a molecular anchor" in Advances in Textile Biotechnology, Vincent Nierstrasz & Artur Cavaco-Paulo, eds., Woodhead Publishing, Cambridge, pp. 266-287. ISBN-13: 978 1 84569 625 2. (Download uncorrected proof).

62. Guce, A.I., Clark, N.E., Salgado, E.N., Ivanen, D.R., Kulminskaya, A.A., Brumer, H., Garman, S.C.* (2010) The catalytic mechanism of human alpha-galactosidase. J. Biol. Chem., 285, 3625-3632. DOI: 10.1074/jbc.M109.060145

61. Gullfot, F., Tan, T.C., von Schantz, L., Nordberg Karlsson, E., Ohlin, M., Brumer, H., and Divne, C.* (2010) The crystal structure of XG-34, an evolved xyloglucan-specific carbohydrate-binding module. Proteins, 78, 785-789. DOI: 10.1002/prot.22642


60. von Schantz, L., Gullfot, F., Scheer, S., Filonova, L., Cicortas Gunnarsson, L., Flint, J.E., Daniel, G., Nordberg-Karlsson, E., Brumer, H., and Ohlin, M.* (2009) Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules. BMC Biotechnol., 9, 92. DOI: 10.1186/1472-6750-9-92

59. Ibatullin, F.M., Banasiak, A., Baumann, M.J., Greffe, L., Takahashi, J., Mellerowicz, E.J. and Brumer, H.* (2009) A real-time fluorogenic assay for the visualization of glycoside hydrolase activity in planta. Plant Physiol., 151, 1741-1750. DOI: 10.1104/pp.109.147439

58. Zhou, Q.,* Brumer, H., and Teeri, T.T. (2009) Self-Organization of cellulose nanocrystals adsorbed with xyloglucan oligosaccharide-poly(ethylene glycol)-polystyrene triblock copolymer. Macromolecules, 42, 5430–5432. DOI: 10.1021/ma901175j

57. Gullfot, F., Ibatullin, F.M., Sundqvist, G., Davies, G.J., and Brumer, H.* (2009) Functional characterization of xyloglucan glycosynthases from GH7, GH12 and GH 16 scaffolds. Biomacromolecules, 10, 1782–1788. DOI: 10.1021/bm900215p

56. Takahashi, J., Rudsander, U.J., Hedenström, M., Banasiak, A., Harholt, J., Amelot, N., Immerzeel, P., Ryden, P., Endo, S., Ibatullin, F.M., Brumer, H., del Campillo, E., Master, E.R., Vibe Scheller, H., Sundberg, B., Teeri, T.T., and Mellerowicz, E.J.* (2009) KORRIGAN1 and its aspen homologue PttCel9A1 decrease cellulose crystallinity in Arabidopsis stem. Plant Cell Physiol., 50, 1099-1115. DOI: 10.1093/pcp/pcp062

55. Eklöf, J.M., Tan, T.C., Divne, C., and Brumer, H.* (2009) The crystal structure of the outer membrane lipoprotein YbhC from Escherichia coli sheds new light on the phylogeny of carbohydrate esterase family 8. Proteins, 76, 1029-1036. DOI: 10.1002/prot.22453

54. Eneyskaya, E.V., Sundqvist, G., Golubev, A.M., Ibatullin, F.M., Ivanen, D.R., Shabalin, K.A., Brumer, H., and Kulminskaya, A.A.* (2009) Transglycosylating and hydrolytic activities of the beta-mannosidase from Trichoderma reesei. Biochimie, 91, 632-638. DOI: 10.1016/j.biochi.2009.03.009

53. Mark, P., Baumann, M.J., Eklöf, J.M., Gullfot, F., Michel, G., Kallas, Å.M., Teeri, T.T., Brumer, H.,* and Czjzek, M.* (2009) Analysis of nasturtium TmNXG1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family GH16 xyloglucan endo-transglycosylases and endo-hydrolases. Proteins, 75, 820-836 DOI: 10.1002/prot.22291


52. Ibatullin, F.M., Baumann, M.J., Greffe, L., Brumer, H.* (2008) Kinetic analyses of retaining endo- (xylo)glucanases from plant and microbial sources using new chromogenic xylogluco-oligosaccharide aryl glycosides. Biochemistry, 47, 7762-7769. DOI: 10.1021/bi8009168

51. Piens, K., Faure, R., Sundqvist, G., Baumann, M.J., Saura-Valls, M., Teeri, T.T., Cottaz, S., Planas, A., Driguez, H., Brumer, H.* (2008) Mechanism-based labelling defines the free energy change for formation of the covalent glycosyl-enzyme intermediate in a xyloglucan endo-transglycosylase. J. Biol. Chem., 283, 21864-21872. DOI: 10.1074/jbc.M803057200

50. Saura-Valls, M., Faure, R., Brumer, H., Teeri, T.T., Cottaz, S., Driguez, H., Planas, A.* (2008) Active-site mapping of a populus xyloglucan endo-transglycosylase with a library of xylogluco-oligosaccharides. J. Biol. Chem., 283, 21853-21863. DOI: 10.1074/jbc.M803058200

49. Gilbert, H.J., Stålbrand, H., Brumer, H.* (2008) How the walls come crumbling down: Recent structural biochemistry of plant polysaccharide degradation. Curr. Opin. Plant Biol., 11, 338-348. DOI: 10.1016/j.pbi.2008.03.004

48. Rudsander, U.J., Sandstrom, C., Piens, K., Master, E.R., Wilson, D.B., Brumer, H., Kenne, L., Teeri, T.T.* (2008) Comparative NMR Analysis of Cellooligosaccharide Hydrolysis by GH9 Bacterial and Plant Endo-1,4-beta-glucanases. Biochemistry, 47, 5235-5241. DOI: 10.1021/bi702193e

47. Nordgren, N., Eklöf, J.M., Zhou, Q., Brumer, H., Rutland, M.W.* (2008) Top-down grafting of xyloglucan to gold monitored by QCM-D and AFM: Enzymatic activity and interactions with cellulose. Biomacromolecules, 9, 942–948. DOI: 10.1021/bm701214e

46. Ahrenstedt, L., Oksanen, A., Salminen, K., Brumer, H.* (2008). Paper dry strength improvement by xyloglucan addition: Wet-end application, spray coating, and synergism with borate. Holzforschung, 62, 8-14. DOI: 10.1515/HF.2008.002


45. Bodin, A., Ahrenstedt, L., Fink, H., Brumer, H., Risberg, B., Gatenholm, P.* (2007). Modification of nanocellulose with a xyloglucan-RGD conjugate enhances adhesion and proliferation of endothelial cells: Implications for tissue engineering. Biomacromolecules, 8, 3697–3704. DOI: 10.1021/bm070343q

44. Piens, K., Henriksson, A.-M., Gullfot, F., Lopez, M., Fauré, R., Ibatullin, F.M., Teeri, T.T., Driguez, H., Brumer, H.* (2007). Glycosynthase activity of hybrid aspen xyloglucan endo-transglycosylase PttXET16-34 nucleophile mutants. Org. Biomol. Chem., 5, 3971-3978. DOI: 10.1039/b714570e

43. Baumann, M.J., Eklöf, J.M., Michel, G., Kallas, Å.M., Teeri, T.T., Czjzek, M.* and Brumer, H.* (2007). Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism. Plant Cell, 19, 1947-1963. DOI: 10.1105/tpc.107.051391 (Recommended in Faculty of 1000 Prime)

42. Teeri, T.T.,* Brumer, H., Daniel, G. and Gatenholm, P. (2007). Biomimetic engineering of cellulose-based materials. Trends Biotech., 25, 299-306. DOI: 10.1016/j.tibtech.2007.05.002

41. Nishikubo, N., Awano, T., Banasiak, A., Bourquin, V., Ibatullin, F., Funada, R., Brumer, H., Teeri, T.T., Hayashi, T., Sundberg, B. and Mellerowicz, E.J.* (2007). Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar - a glimpse into the mechanism of the balancing act of trees. Plant Cell Physiol., 48, 843-855. DOI: 10.1093/pcp/pcm055

40. Gloster, T.M., Ibatullin, F.M., Macauley, K., Eklöf, J.M., Roberts, S., Turkenburg, J.P., Bjørnvad, M.E., Linå Jørgensen, P., Danielsen, S., Johansen, K.S., Borchert, T.V., Wilson, K.S., Brumer, H.,* and Davies, G.J.* (2007). Characterisation and 3-D structures of two distinct bacterial xyloglucanases from families GH5 and GH12. J. Biol. Chem., 282, 19177-19189. DOI: 10.1074/jbc.M700224200

39. Zhou, Q., Rutland, M.W., Teeri, T.T., and Brumer, H.* (2007). Xyloglucan in cellulose modification. Cellulose, 14, 625-641. DOI: 10.1007/s10570-007-9109-0

38. Sundqvist, G., Stenvall, M., Berglund, H., Ottosson, J., and Brumer, H.* (2007). A general, robust method for the quality control of intact proteins using LC-ESI-MS. J. Chrom. B, 852, 188-194. DOI: 10.1016/j.jchromb.2007.01.011

37. Comfort, D.A., Bobrov, K.S., Ivanen, D.R., Shabalin, K.A., Harris, J.M., Kulminskaya, A.A., Brumer, H., Kelly, R.M.* (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry, 46, 3319-3330. DOI: 10.1021/bi061521n


36. Neustroev, K.N., Golubev, A.M., Sinnott, M.L., Borriss, R., Krah, M., Brumer, H., Eneyskaya, E.V., Shishlyannikov, S., Shabalin, K.A., Peshechonov, V.T., Korolev, V.G., Kulminskaya, A.A.* (2006). Transferase and hydrolytic activities of the laminarinase from Rhodothermus marinus and its M133A, M133C, and M133W mutants. Glycoconj. J., 23, 501-511. DOI: 10.1007/s10719-006-6733-0

35. Stiernstedt, J., Nordgren, N., Wågberg, L., Brumer, H., Gray, D.G., and Rutland, M.W.* (2006). Friction and forces between cellulose model surfaces: a comparison. J. Coll. Int. Sci., 303, 117-123. DOI: 10.1016/j.jcis.2006.06.070

34. Cicortas Gunnarsson, L., Zhou, Q., Montanier, C., Nordberg Karlsson, E., Brumer, H., and Ohlin, M.* (2006). Engineered xyloglucan specificity in a carbohydrate-binding module. Glycobiology, 16, 1171-1180. DOI: 10.1093/glycob/cwl038 (Recommended in Faculty of 1000 Prime)

33. Lönnberg, H., Zhou, Q., Brumer, H., Teeri, T., Malmström, E., and Hult, A.* (2006). Grafting of Cellulose Fibers with Poly(epsilon-caprolactone) and Poly(L-lactic acid) via Ring-Opening Polymerization. Biomacromolecules, 7, 2178-2185. DOI: 10.1021/bm060178z

32. Stiernstedt, J., Brumer, H., Zhou, Q., Teeri, T.T. and Rutland M.W.* (2006). Friction between Cellulose Surfaces and Effect of Xyloglucan Adsorption. Biomacromolecules, 7, 2147-2153. DOI: 10.1021/bm060100i

31. Zhou, Q., Baumann, M.J., Piispanen, P.S., Teeri, T.T., and Brumer, H.* (2006). Xyloglucan and xyloglucan endo-transglycosylases (XET): Tools for ex vivo cellulose surface modification. Biocatal. Biotransform., 24, 107-120. DOI: 10.1080/10242420500538217

30. Zhou, Q., Baumann, M.J., Brumer, H.*, and Teeri, T.T. (2006). The influence of surface chemical composition on the adsorption of xyloglucan to chemical and mechanical pulps. Carbohydr. Polym., 63, 449-458. DOI: 10.1016/j.carbpol.2005.09.015

29. Faure, R., Saura-Valls, M., Brumer, H., Planas, A., Cottaz, S., and Driguez, H.* (2006). Synthesis of a Library of Xylogluco-Oligosaccharides for Active-Site Mapping of Xyloglucan endo-Transglycosylase. J. Org. Chem., 71, 5151-5161. DOI: 10.1021/jo0525682

28. Martinez-Fleites, C., Guerreiro, C.I., Baumann, M.J., Taylor, E.J., Prates, J.A., Ferreira, L.M., Fontes, C.M., Brumer, H., and Davies, G.J.* (2006). Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation. J. Biol. Chem., 281, 24922-24933. DOI: 10.1074/jbc.M603583200

27. Saura-Valls, M., Faure, R., Ragas, S., Piens, K., Brumer, H., Teeri, T.T., Cottaz, S., Driguez, H., and Planas, A.* (2006). Kinetic analysis using low-molecular mass xyloglucan oligosaccharides defines the catalytic mechanism of a Populus xyloglucan endotransglycosylase. Biochem. J., 395, 99-106. DOI: 10.1042/BJ20051396


26. Uhlén, M.* et al. (2005). A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol. Cell. Proteomics, 4, 1920-1932. DOI: 10.1074/mcp.M500279-MCP200

25. Kallas, Å.M., Piens, K., Denman, S., Henriksson, H., Fäldt, J., Johansson, P., Brumer, H., Teeri, T.T.* (2005). Enzymatic properties of native and deglycosylated hybrid aspen (Populus tremula x tremuloides) xyloglucan endotransglycosylase 16A expressed in Pichia pastoris. Biochem. J., 390, 105-113. DOI: 10.1042/BJ20041749

24. Ghebremichael, K.A., Gunaratna, K.R., Henriksson, H., Brumer, H., and Dalhammar, G.* (2005). A simple purification and activity assay of the coagulant protein from Moringa oleifera seed. Water Res., 39, 2338-2344. DOI: 10.1016/j.watres.2005.04.012

23. Engfeldt, T., Renberg, B., Brumer, H., Nygren, P.-Å., and Eriksson Karlström, A.* (2005). Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein. ChemBioChem, 6, 1043-1050. DOI: 10.1002/cbic.200400388

22. Zhou, Q., Greffe, L., Baumann, M.J., Malmström, E., Teeri, T.T.,and Brumer, H.* (2005). The use of xyloglucan as a molecular anchor for the elaboration of polymers from cellulose surfaces: a general route for the design of biocomposites. Macromolecules, 38, 3547-3549. DOI: 10.1021/ma047712k

21. Greffe L., Bessueille L., Bulone V., and Brumer H.* (2005). Synthesis, preliminary characterization, and application of novel surfactants from highly branched xyloglucan oligosaccharides. Glycobiology, 15, 437-445. DOI: 10.1093/glycob/cwi013

20. Gustavsson M.T., Persson P.V., Iversen T., Martinelle M., Hult K., Teeri T.T., and Brumer, H.* (2005). Modification of cellulose fiber surfaces by use of a lipase and a xyloglucan endotransglycosylase. Biomacromolecules, 6, 196-203. DOI: 10.1021/bm049588i

19. Eneyskaya, E.V., Ivanen, D.R., Shabalin, K.A., Kulminskaya, A.A., Backinowsky, L.V., Brumer, H., and Neustroev, K.N.* (2005). Chemo-enzymatic synthesis of 4-methylumbelliferyl beta (1-4)-D-xylooligosides: new substrates for beta-D-xylanase assays. Org. Biomol. Chem., 3, 146-151. DOI: 10.1039/b409583a


18. Johansson, P., Brumer, H., Baumann, M. J., Kallas, A. M., Henriksson, H., Denman, S. E., Teeri, T. T. and Jones, T. A.* (2004). Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding. Plant Cell, 16, 874-886. DOI: 10.1105/tpc.020065 (Recommended in Faculty of 1000 Prime)

17. Eriksson, T., Stals, I., Collen, A., Tjerneld, F., Claeyssens, M., Stalbrand, H. and Brumer, H.* (2004). Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reeseii) Cel7B catalytic module. Eur. J. Biochem., 271, 1266-1276. DOI: 10.1111/j.1432-1033.2004.04031.x

16. Brumer, H., Zhou, Q., Baumann, M. J., Carlsson, K. and Teeri, T. T.* (2004). Activation of crystalline cellulose surfaces through the chemoenzymatic modification of xyloglucan. J. Am. Chem. Soc., 126, 5715-5721. DOI: 10.1021/ja0316770


15. Teeri, T. T. and Brumer, H.* (2003). Discovery, characterisation and applications of enzymes from the wood-forming tissues of poplar: Glycosyl transferases and xyloglucan endotransglycosylases. Biocatal. Biotransform., 21, 173-179. DOI: 10.1080/1024-2420310001618492

14. Henriksson, H., Denman, S., Campuzano, I., Master, E., Ademark, P., Teeri, T.T., and Brumer, H.* (2003). N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A. Biochem. J., 375, 61-73. DOI: 10.1042/BJ20030485

13. Christiernin, M., Henriksson, G., Lindstrom, M.E., Brumer, H., Teeri, T.T., Lindstrom, T.*, and Laine, J.* (2003). The effects of xyloglucan on the properties of paper made from bleached kraft pulp. Nord. Pulp Paper Res. J., 18, 182-187. DOI: 10.3183/NPPRJ-2003-18-02-p182-187

12. Borriss, R., Krah, M., Brumer, H., Kerzhner, M.A., Ivanen, D.R., Eneyskaya, E.V., Elyakova, L.A., Shishlyannikov, S.M., Shabalin, K.A., and Neustroev, K.N.* (2003). Enzymatic synthesis of 4-methylumbelliferyl (1-3)-beta-D-glucooligosaccharides - new substrates for beta-1,3-1,4-D-glucanase. Carbohydrate Res., 338, 1455-67. DOI: 10.1016/S0008-6215(03)00199-X

11. Johansson, P., Denman, S., Brumer, H., Kallas, Å.M., Henriksson, H., Bergfors, T., Teeri, T.T., and Jones, T.A.* (2003). Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides. Acta Crystallogr. Sect. D, 59, 535-537. DOI: 10.1107/S090744490202348X

10. Eneyskaya, E.V., Brumer, H., Backinowsky, L.V., Ivanen, D.R., Kulminskaya, A.A., Shabalin, K.A., and Neustroev, K.N.* (2003). Enzymatic synthesis of beta-xylanase substrates: transglycosylation reactions of the beta-xylosidase from Aspergillus sp. Carbohydrate Res., 338, 313-325. DOI: 10.1016/S0008-6215(02)00467-6


9. Brumer, H., "Carbohydrases" in The Encyclopedia of Catalysis, István T. Horváth, ed., John Wiley and Sons, New York, 2002. DOI: 10.1002/0471227617.eoc037

8. Bourquin, V., Nishikubo, N., Abe, H., Brumer, H., Denman, S., Eklund, M., Christiernin, M., Teeri, T.T., Sundberg, B., and Mellerowicz, E.J.* (2002). Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues. Plant Cell, 14, 3073-3088. DOI: 10.1105/tpc.007773


7. Brun, E., Brumer, H., MacKenzie, L.F., Withers, S.G.,* McIntosh, L.P. (2001). Letter to the Editor: Assignment of selectively C-13-labeled cellopentaose synthesized using an engineered glycosynthase. J. Biomol. NMR, 21, 67-68. DOI: 10.1023/A:1011927400439

6. Becker, D., Braet, C., Brumer, H., Claeyssens, M., Divne, C., Fagerstrom, B.R., Harris, M., Jones, T.A., Kleywegt, G.J., Koivula, A., Mahdi, S., Piens, K., Sinnott, M.L.,* Stahlberg, J., Teeri, T.T., Underwood, M., Wohlfahrt, G. (2001). Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei CeI7A and its E223S/A224H/L225V/T226A/D262G mutant. Biochem. J., 356, 19-30.


5. Hart, D.O., He, S., Chany II, C.J., Withers, S.G., Sims, P.F.G., Sinnott, M.L., and Brumer, H.* (2000). Identification of Asp-130 as the catalytic nucleophile in the main alpha-galactosidase from Phanerochaete chrysosporium, a family 27 glycosyl hydrolase. Biochemistry, 39, 9826-9836. DOI: 10.1021/bi0008074


4. Brumer, H., Sims, P.F.G., and Sinnott, M.L.* (1999). Lignocellulose degradation by Phanerochaete chrysosporium: purification and characterisation of the main alpha-galactosidase. Biochem. J., 339, 43-53.


3. Moriarty, R.M.* and Brumer, H. (1995). Toward a general synthesis of A-ring trihydroxylated vitamin D analogs: synthesis of an A-ring synthon of ED-71 from D-arabinose. Tetrahedron Lett., 36, 9265-9268. DOI: 10.1016/0040-4039(95)02008-D

2. Moriarty, R.M.,* Booth, D.T., and Brumer, H. (1995). Palladium catalyzed alkylative cyclization of iodonium salts with enynes: a mild and stereoselective reaction using hypervalent iodine. Abstracts of Papers of the Amer. Chem. Soc., 210, 397.

1. Moriarty, R.M.,* Kim, J., and Brumer, H. (1995). A general synthetic route to A-ring hydroxylated vitamin D analogs from pentoses. Tetrahedron Lett., 36, 51-54. DOI: 10.1016/0040-4039(94)02209-T


This section includes patent applications and granted patents.

6. Ståhl, P., Brumer, H., Song, Y., de Araújo Silva, A.C., Lundeberg, J. (2011) Surface-functionalized cellulosic fibres, method of manufacture thereof and uses thereof. WO2013072408.

5. Slättegård, R., Ruda, M., Brumer, H. (2009) Aminated hemicellulose molecule and method for production thereof. WO2010090591

4. Bodin, A., Gatenholm, P., Fink, H., Risberg, B., Brumer, H., Ahrenstedt, N.L. (2008) Implantable material for medical or surgical applications. WO2008104528.

3. Zhou, Q., Brumer, H., Teeri, T.T., Stolt, J.P., Ödberg, L.G. (2007) Copolymer, modified polymer carbohydrate material, modified build polymer, composite material, and methods of preparation WO2007149045.

2. Brumer, H., Rutland, M.W., Sinnott, M.L., Teeri, T.T., Zhou, Q. (2006). Cross-linking involving a polymeric carbohydrate material. WO2006079512

1. Teeri, T.T. and Brumer, H. (2003). Enzymatic method for the modification of polymeric carbohydrate materials. WO 03/033813


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Learning from microbial strategies for polysaccharide degradation