Our lab is studies a variety of host-pathogen combinations. We mostly use quantitative proteomics, employing stable isotope labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) but also use other high-throughput methods, such as high content screening and massively parallel sequencing. To fill in the gaps we then turn to more focused biochemical and cell biological methods. Recently we have described a novel method for mapping the protein interaction network within cells (the interactome) that drives the time and cost involved in such an analysis down by nearly two orders of magnitude; this technique will play an important role in many current and future projects.
- Mapping signaling pathways downstream of and disrupted by Salmonella invasion
- Finding host targets of effector proteins secreted from pathogenic bacteria
- Mapping protein interaction networks using protein correlation profiling and SILAC
- Exploring the relationship between polymorphism in the antigen presentation machinery of humans and the antigens that are ultimately presented
- Identifying biomarkers of disease resistance in honey bees
- Understanding the molecular mechanisms of pathogenesis in viral infections of honey bees
Pubmed Search link
Imami, K., A.P. Bhavsar, Y. Hongbing, N.F. Brown, L.D. Rogers, B.B. Finlay and L.J. Foster. “Global impact of Salmonella pathogenicity island 2-secreted effectors on the host phosphoproteome.” MOLECULAR & CELLULAR PROTEOMICS.
Kristensen, A.R., J. Gsponer and L.J. Foster. “A high-throughput approach for measuring temporal changes in the interactome.” NATURE METHODS. 9.9 (2012): 907 – 909.
Parker, P., M.M. Guarna, A.P. Melathopoulos, K.-M. Moon, R. White, E. Huxter, S.F. Pernal and L.J. Foster. “Correlation of proteome-wide changes with social immunity behaviors reveals mechanisms underpinning phenotypic adaptation of the honey bee (Apis mellifera) to disease.” GENOME BIOLOGY. 13.9 (2012): R81.
Rogers, L.D., Y. Fang, N.F. Brown, S. Pelech and L.J. Foster. “SopB is a master regulator of host signaling cascades during Salmonella infection.” SCIENCE SIGNALING. 4.191 (2011): rs9.
Zheng, Y.Z., C. Boscher, K.L. Inder, M. Fairbank, D. Loo, M.M. Hill, I.R. Nabi and L.J. Foster. “Differential impact of caveolae and caveolin-1 scaffolds on the membrane raft proteome.” MOLECULAR & CELLULAR PROTEOMICS. 10.10 (2011): M110.007146.
Chan, Q.W.T., S. Cornman, N. Liao, S. Chan, R. Docking, G. Taylor, S. Jones, D.C. de Graaf, J.D. Evans and L.J. Foster. “Updated genome assembly and annotation of Paenibacillus larvae, the agent of American Foulbrood disease of honey bees.” BMC GENOMICS. 12.1 (2011): 450.
Chan, Q.W.T., R. Parker, Z. Sun, E.W. Deutsch* and L.J. Foster*. “A honey bee (Apis mellifera L.) PeptideAtlas crossing castes and tissues.” BMC GENOMICS. 12.1 (2011): 290. *Shared corresponding authorship.
Auweter, S.D., A.P. Bhavsar, C.L. de Hoog, Y. Li, Y.A. Chan, M.J. Lowden, L.D. Rogers, N. Stoynov, L.J. Foster* and B.B. Finlay*. “Using quantitative mass spectrometry to catalog Salmonella Pathogenicity Island-2 effectors and identify their cognate host binding partners.” JOURNAL OF BIOLOGICAL CHEMISTRY.286.27 (2011): 24023 – 24035. *Shared corresponding authorship.
Foster, L.J. “Interpretation of data underlying the link between CCD and an invertebrate iridescent virus.” MOLECULAR & CELLULAR PROTEOMICS. 10.3 (2011): M110.00687.
Rechavi, O., M. Kalman, Y. Fang, H. Vernitsky, J. Jacob-Hirsch, L.J.Foster, Y. Kloog and I. Goldstein. “Trans-SILAC: sorting out the Non-Cell-Autonomous Proteome.” NATURE METHODS. 7.11 (2010): 923 – 927.
Parker, R., A.P. Melathopoulos, R. White, S.F. Pernal, M.M. Guarna and L.J. Foster. “Ecological Adaptation of Diverse Honey Bee (Apis mellifera) Populations.” PLOS ONE. 5.6 (2010): e11096.
Fang, Y., D.P. Robinson and L.J. Foster. “Quantitative analysis of yield and recovery for upstream fractionation methods in proteomics.” JOURNAL OF PROTEOME RESEARCH. 9.4 (April 2010): 1902 – 1912.
Rogers, L.D. and L.J. Foster. “The dynamic phagosome proteome and the contribution of the ER”. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE U.S.A. 104.47 (2007): 18520 – 18525.